2c5s

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[[Image:2c5s.png|left|200px]]
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==CRYSTAL STRUCTURE OF BACILLUS ANTHRACIS THII, A TRNA-MODIFYING ENZYME CONTAINING THE PREDICTED RNA-BINDING THUMP DOMAIN==
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<StructureSection load='2c5s' size='340' side='right' caption='[[2c5s]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2c5s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C5S FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene><br>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c5s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c5s RCSB], [http://www.ebi.ac.uk/pdbsum/2c5s PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c5/2c5s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ThiI is an enzyme responsible for the formation of the modified base S(4)U (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This base acts as a sensitive trigger for the response mechanism to UV exposure, providing protection against its damaging effects. We present the crystal structure of Bacillus anthracis ThiI in complex with AMP, revealing an extended tripartite architecture in which an N-terminal ferredoxin-like domain (NFLD) connects the C-terminal catalytic PP-loop pyrophosphatase domain with a THUMP domain, an ancient predicted RNA-binding domain that is widespread in all kingdoms of life. We describe the structure of the THUMP domain, which appears to be unrelated to RNA-binding domains of known structure. Mapping the conserved residues of NFLD and the THUMP domain onto the ThiI structure suggests that these domains jointly form the tRNA-binding surface. The inaccessibility of U8 in the canonical L-shaped form of tRNA, and the existence of a glycine-rich linker joining the catalytic and RNA-binding moieties of ThiI suggest that structural changes may occur in both molecules upon binding.
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{{STRUCTURE_2c5s| PDB=2c5s | SCENE= }}
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Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain.,Waterman DG, Ortiz-Lombardia M, Fogg MJ, Koonin EV, Antson AA J Mol Biol. 2006 Feb 10;356(1):97-110. Epub 2005 Nov 22. PMID:16343540<ref>PMID:16343540</ref>
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===CRYSTAL STRUCTURE OF BACILLUS ANTHRACIS THII, A TRNA-MODIFYING ENZYME CONTAINING THE PREDICTED RNA-BINDING THUMP DOMAIN===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_16343540}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2c5s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5S OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:016343540</ref><references group="xtra"/>
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[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
[[Category: Antson, A A.]]
[[Category: Antson, A A.]]

Revision as of 01:12, 30 September 2014

CRYSTAL STRUCTURE OF BACILLUS ANTHRACIS THII, A TRNA-MODIFYING ENZYME CONTAINING THE PREDICTED RNA-BINDING THUMP DOMAIN

2c5s, resolution 2.50Å

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