This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1gvn
From Proteopedia
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
| - | Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the .. | + | Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the ATPGTP-binding site. To our knowledge, this is the, first prokaryotic postsegregational killing system that has been entirely, structurally characterized. |
==About this Structure== | ==About this Structure== | ||
| - | 1GVN is a | + | 1GVN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: SOB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVN OCA]. |
==Reference== | ==Reference== | ||
| Line 22: | Line 22: | ||
[[Category: postsegregational killing system]] | [[Category: postsegregational killing system]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:45:06 2007'' |
Revision as of 12:39, 5 November 2007
|
CRYSTAL STRUCTURE OF THE PLASMID MAINTENANCE SYSTEM EPSILON/ZETA: MEACHNISM OF TOXIN INACTIVATION AND TOXIN FUNCTION
Overview
Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the ATPGTP-binding site. To our knowledge, this is the, first prokaryotic postsegregational killing system that has been entirely, structurally characterized.
About this Structure
1GVN is a Protein complex structure of sequences from Streptococcus pyogenes with SO4 as ligand. Structure known Active Site: SOB. Full crystallographic information is available from OCA.
Reference
Crystal structure of the plasmid maintenance system epsilon/zeta: functional mechanism of toxin zeta and inactivation by epsilon 2 zeta 2 complex formation., Meinhart A, Alonso JC, Strater N, Saenger W, Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1661-6. Epub 2003 Feb 5. PMID:12571357
Page seeded by OCA on Mon Nov 5 14:45:06 2007
