2gim

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[[Image:2gim.png|left|200px]]
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==1.6 Angstrom structure of plastocyanin from Anabaena variabilis==
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<StructureSection load='2gim' size='340' side='right' caption='[[2gim]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gim]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis Anabaena variabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GIM FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1172 Anabaena variabilis])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gim OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gim RCSB], [http://www.ebi.ac.uk/pdbsum/2gim PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gi/2gim_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.
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{{STRUCTURE_2gim| PDB=2gim | SCENE= }}
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Structure of plastocyanin from the cyanobacterium Anabaena variabilis.,Schmidt L, Christensen HE, Harris P Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103<ref>PMID:16929103</ref>
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===1.6 Angstrom structure of plastocyanin from Anabaena variabilis===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_16929103}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2gim]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis Anabaena variabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:016929103</ref><references group="xtra"/>
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[[Category: Anabaena variabilis]]
[[Category: Anabaena variabilis]]
[[Category: Christensen, H E.M.]]
[[Category: Christensen, H E.M.]]

Revision as of 08:30, 30 September 2014

1.6 Angstrom structure of plastocyanin from Anabaena variabilis

2gim, resolution 1.60Å

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