2hps

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[[Image:2hps.png|left|200px]]
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==Crystal structure of coelenterazine-binding protein from Renilla Muelleri==
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<StructureSection load='2hps' size='340' side='right' caption='[[2hps]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hps]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_muelleri Renilla muelleri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HPS FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTZ:C2-HYDROXY-COELENTERAZINE'>CTZ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hps RCSB], [http://www.ebi.ac.uk/pdbsum/2hps PDBsum], [http://www.topsan.org/Proteins/SECSG/2hps TOPSAN]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/2hps_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bioluminescence in the sea pansy Renilla involves two distinct proteins, a Ca2+-triggered coelenterazine-binding protein (CBP), and Renilla luciferase. CBP contains one tightly bound coelenterazine molecule, which becomes available for reaction with luciferase and O2 only subsequent to Ca2+ binding. CBP belongs to the EF-hand superfamily of Ca2+-binding proteins and contains three "EF-hand" Ca2+-binding sites. The overall spatial structure of recombinant selenomethionine-labeled CBP determined at 1.7 A, is found to approximate the protein scaffold characteristic of the class of Ca2+-regulated photoproteins. Photoproteins however, catalyze molecular oxygen addition to coelenterazine producing a 2-hydroperoxycoelenterazine intermediate, which is stabilized within the binding cavity in the absence of Ca2+. Addition of Ca2+ triggers the bioluminescence reaction. However in CBP this first step of oxygen addition is not allowed. The different amino acid environments and hydrogen bond interactions within the binding cavity, are proposed to account for the different properties of the two classes of proteins.
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{{STRUCTURE_2hps| PDB=2hps | SCENE= }}
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Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: why it is not a calcium-regulated photoprotein.,Stepanyuk GA, Liu ZJ, Markova SS, Frank LA, Lee J, Vysotski ES, Wang BC Photochem Photobiol Sci. 2008 Apr;7(4):442-7. Epub 2008 Feb 19. PMID:18385886<ref>PMID:18385886</ref>
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===Crystal structure of coelenterazine-binding protein from Renilla Muelleri===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18385886}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2hps]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_muelleri Renilla muelleri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HPS OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:018385886</ref><references group="xtra"/>
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[[Category: Renilla muelleri]]
[[Category: Renilla muelleri]]
[[Category: Lee, J.]]
[[Category: Lee, J.]]

Revision as of 08:46, 30 September 2014

Crystal structure of coelenterazine-binding protein from Renilla Muelleri

2hps, resolution 1.72Å

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