1vzi

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[[Image:1vzi.gif|left|200px]]<br /><applet load="1vzi" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1vzi.gif|left|200px]]
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caption="1vzi, resolution 1.15&Aring;" />
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'''STRUCTURE OF SUPEROXIDE REDUCTASE BOUND TO FERROCYANIDE AND ACTIVE SITE EXPANSION UPON X-RAY INDUCED PHOTOREDUCTION'''<br />
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{{Structure
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|PDB= 1vzi |SIZE=350|CAPTION= <scene name='initialview01'>1vzi</scene>, resolution 1.15&Aring;
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|SITE= <scene name='pdbsite=AC1:Fe2+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=FE2:FE (II) ION'>FE2</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Superoxide_reductase Superoxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.2 1.15.1.2]
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|GENE=
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}}
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'''STRUCTURE OF SUPEROXIDE REDUCTASE BOUND TO FERROCYANIDE AND ACTIVE SITE EXPANSION UPON X-RAY INDUCED PHOTOREDUCTION'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1VZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_baarsii Desulfovibrio baarsii] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=FE2:'>FE2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Superoxide_reductase Superoxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.2 1.15.1.2] Known structural/functional Site: <scene name='pdbsite=AC1:Fe2+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZI OCA].
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1VZI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_baarsii Desulfovibrio baarsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZI OCA].
==Reference==
==Reference==
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Structure of superoxide reductase bound to ferrocyanide and active site expansion upon X-ray-induced photo-reduction., Adam V, Royant A, Niviere V, Molina-Heredia FP, Bourgeois D, Structure. 2004 Sep;12(9):1729-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15341736 15341736]
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Structure of superoxide reductase bound to ferrocyanide and active site expansion upon X-ray-induced photo-reduction., Adam V, Royant A, Niviere V, Molina-Heredia FP, Bourgeois D, Structure. 2004 Sep;12(9):1729-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341736 15341736]
[[Category: Desulfovibrio baarsii]]
[[Category: Desulfovibrio baarsii]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
[[Category: photoreduction]]
[[Category: photoreduction]]
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[[Category: redox states]]
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[[Category: redox state]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:38:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:50:07 2008''

Revision as of 12:50, 20 March 2008

Image:1vzi.gif


PDB ID 1vzi

Drag the structure with the mouse to rotate
, resolution 1.15Å
Sites:
Ligands: , and
Activity: Superoxide reductase, with EC number 1.15.1.2
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF SUPEROXIDE REDUCTASE BOUND TO FERROCYANIDE AND ACTIVE SITE EXPANSION UPON X-RAY INDUCED PHOTOREDUCTION


Overview

Some sulfate-reducing and microaerophilic bacteria rely on the enzyme superoxide reductase (SOR) to eliminate the toxic superoxide anion radical (O2*-). SOR catalyses the one-electron reduction of O2*- to hydrogen peroxide at a nonheme ferrous iron center. The structures of Desulfoarculus baarsii SOR (mutant E47A) alone and in complex with ferrocyanide were solved to 1.15 and 1.7 A resolution, respectively. The latter structure, the first ever reported of a complex between ferrocyanide and a protein, reveals that this organo-metallic compound entirely plugs the SOR active site, coordinating the active iron through a bent cyano bridge. The subtle structural differences between the mixed-valence and the fully reduced SOR-ferrocyanide adducts were investigated by taking advantage of the photoelectrons induced by X-rays. The results reveal that photo-reduction from Fe(III) to Fe(II) of the iron center, a very rapid process under a powerful synchrotron beam, induces an expansion of the SOR active site.

About this Structure

1VZI is a Single protein structure of sequence from Desulfovibrio baarsii. Full crystallographic information is available from OCA.

Reference

Structure of superoxide reductase bound to ferrocyanide and active site expansion upon X-ray-induced photo-reduction., Adam V, Royant A, Niviere V, Molina-Heredia FP, Bourgeois D, Structure. 2004 Sep;12(9):1729-40. PMID:15341736

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