2hw0

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[[Image:2hw0.png|left|200px]]
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==NMR Solution Structure of the nuclease domain from the Replicator Initiator Protein from porcine circovirus PCV2==
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<StructureSection load='2hw0' size='340' side='right' caption='[[2hw0]], [[NMR_Ensembles_of_Models | 31 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Porcine_circovirus_2 Porcine circovirus 2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HW0 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rep, ORF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85708 Porcine circovirus 2])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hw0 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/2hw0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Circoviruses are the smallest circular single-stranded DNA viruses able to replicate in mammalian cells. Essential to their replication is the replication initiator, or Rep protein that initiates the rolling circle replication (RCR) of the viral genome. Here we report the NMR solution three-dimensional structure of the endonuclease domain from the Rep protein of porcine circovirus type 2 (PCV2), the causative agent of postweaning multisystemic wasting syndrome in swine. The domain comprises residues 12-112 of the full-length protein and exhibits the fold described previously for the Rep protein of the representative geminivirus tomato yellow leaf curl Sardinia virus. The structure, however, differs significantly in some secondary structure elements that decorate the central five-stranded beta-sheet, including the replacement of a beta-hairpin by an alpha-helix in PCV2 Rep. The identification of the divalent metal binding site was accomplished by following the paramagnetic broadening of NMR amide signals upon Mn(2+) titration. The site comprises three conserved acidic residues on the exposed face of the central beta-sheet. For the 1:1 complex of the PCV2 Rep nuclease domain with a 22mer double-stranded DNA oligonucleotide chemical shift mapping allowed the identification of the DNA binding site on the protein and aided in constructing a model of the protein/DNA complex.
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{{STRUCTURE_2hw0| PDB=2hw0 | SCENE= }}
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Solution structure, divalent metal and DNA binding of the endonuclease domain from the replication initiation protein from porcine circovirus 2.,Vega-Rocha S, Byeon IJ, Gronenborn B, Gronenborn AM, Campos-Olivas R J Mol Biol. 2007 Mar 23;367(2):473-87. Epub 2007 Jan 9. PMID:17275023<ref>PMID:17275023</ref>
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===NMR Solution Structure of the nuclease domain from the Replicator Initiator Protein from porcine circovirus PCV2===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_17275023}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2hw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Porcine_circovirus_2 Porcine circovirus 2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HW0 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:017275023</ref><references group="xtra"/>
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[[Category: Porcine circovirus 2]]
[[Category: Porcine circovirus 2]]
[[Category: Byeon, I L.]]
[[Category: Byeon, I L.]]

Revision as of 09:13, 30 September 2014

NMR Solution Structure of the nuclease domain from the Replicator Initiator Protein from porcine circovirus PCV2

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