2fft

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2fft" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2fft.png|left|200px]]
+
==NMR structure of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa in SDS Micelles==
 +
<StructureSection load='2fft' size='340' side='right' caption='[[2fft]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2fft]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FFT FirstGlance]. <br>
 +
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TSP9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3562 Spinacia oleracea])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fft OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fft RCSB], [http://www.ebi.ac.uk/pdbsum/2fft PDBsum], [http://www.topsan.org/Proteins/CESG/2fft TOPSAN]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Thylakoid soluble phosphoprotein of 9 kDa (TSP9) has been identified as a plant-specific protein in the photosynthetic thylakoid membrane (Carlberg et al. (2003) Proc. Natl. Acad. Sci. 100, 757-762). Nonphosphorylated TSP9 is associated with the membrane, whereas, after light-induced phosphorylation, a fraction of the phosphorylated TSP9 is released into the aqueous stroma. By NMR spectroscopy, we have determined the structural features of nonphosphorylated TSP9 both in aqueous solution and in membrane mimetic micelles. The results show that both wild type nonphosphorylated TSP9 and a triple-mutant (T46E + T53E + T60E) mimic of the triphosphorylated form of TSP9 are disordered under aqueous conditions, but adopt an ordered conformation in the presence of detergent micelles. The micelle-induced structural features, which are similar in micelles either of SDS or dodecylphosphocholine (DPC), consist of an N-terminal alpha-helix, which may represent the primary site of interaction between TSP9 and binding partners, and a less structured helical turn near the C-terminus. These structured elements contain mainly hydrophobic residues. NMR relaxation data for nonphosphorylated TSP9 in SDS micelles indicated that the molecule is highly flexible with the highest order in the N-terminal alpha-helix. Intermolecular NOE signals, as well as spin probe-induced broadening of NMR signals, demonstrated that the SDS micelles contact both the structured and a portion of the unstructured regions of TSP9, in particular, those containing the three phosphorylation sites (T46, T53, and T60). This interaction may explain the selective dissociation of phosphorylated TSP9 from the membrane. Our study presents a structural model for the role played by the structured and unstructured regions of TSP9 in its membrane association and biological function.
-
{{STRUCTURE_2fft| PDB=2fft | SCENE= }}
+
Micelle-induced folding of spinach thylakoid soluble phosphoprotein of 9 kDa and its functional implications.,Song J, Lee MS, Carlberg I, Vener AV, Markley JL Biochemistry. 2006 Dec 26;45(51):15633-43. Epub 2006 Dec 8. PMID:17176085<ref>PMID:17176085</ref>
-
===NMR structure of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa in SDS Micelles===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_17176085}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[2fft]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFT OCA].
+
</StructureSection>
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:017176085</ref><references group="xtra"/>
+
[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
[[Category: CESG, Center for Eukaryotic Structural Genomics.]]

Revision as of 15:33, 12 October 2014

NMR structure of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa in SDS Micelles

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fft"
Personal tools