1w36
From Proteopedia
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| - | [[Image:1w36.gif|left|200px]] | + | [[Image:1w36.gif|left|200px]] |
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| - | '''RECBCD:DNA COMPLEX''' | + | {{Structure |
| + | |PDB= 1w36 |SIZE=350|CAPTION= <scene name='initialview01'>1w36</scene>, resolution 3.1Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+E'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_V Exodeoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.5 3.1.11.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''RECBCD:DNA COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W36 is a [ | + | 1W36 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W36 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks., Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB, Nature. 2004 Nov 11;432(7014):187-93. PMID:[http:// | + | Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks., Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB, Nature. 2004 Nov 11;432(7014):187-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15538360 15538360] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Exodeoxyribonuclease V]] | [[Category: Exodeoxyribonuclease V]] | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:51:34 2008'' |
Revision as of 12:51, 20 March 2008
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| , resolution 3.1Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Exodeoxyribonuclease V, with EC number 3.1.11.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RECBCD:DNA COMPLEX
Overview
RecBCD is a multi-functional enzyme complex that processes DNA ends resulting from a double-strand break. RecBCD is a bipolar helicase that splits the duplex into its component strands and digests them until encountering a recombinational hotspot (Chi site). The nuclease activity is then attenuated and RecBCD loads RecA onto the 3' tail of the DNA. Here we present the crystal structure of RecBCD bound to a DNA substrate. In this initiation complex, the DNA duplex has been split across the RecC subunit to create a fork with the separated strands each heading towards different helicase motor subunits. The strands pass along tunnels within the complex, both emerging adjacent to the nuclease domain of RecB. Passage of the 3' tail through one of these tunnels provides a mechanism for the recognition of a Chi sequence by RecC within the context of double-stranded DNA. Gating of this tunnel suggests how nuclease activity might be regulated.
About this Structure
1W36 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks., Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB, Nature. 2004 Nov 11;432(7014):187-93. PMID:15538360
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