This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5lip
From Proteopedia
(New page: 200px<br /> <applet load="5lip" size="450" color="white" frame="true" align="right" spinBox="true" caption="5lip, resolution 2.9Å" /> '''PSEUDOMONAS LIPASE C...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 5LIP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]] with CA and OCP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5LIP OCA]]. | + | 5LIP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]] with CA and OCP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]]. Structure known Active Sites: ACT and OXY. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5LIP OCA]]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 24: | ||
[[Category: triglyceride analogue]] | [[Category: triglyceride analogue]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:34:56 2007'' |
Revision as of 06:30, 30 October 2007
|
PSEUDOMONAS LIPASE COMPLEXED WITH RC-(RP, SP)-1,2-DIOCTYLCARBAMOYLGLYCERO-3-O-OCTYLPHOSPHONATE
Overview
To investigate the enantioselectivity of Pseudomonas cepacia lipase, inhibition studies were performed with Sc- and, Rc-(Rp,Sp)-1,2-dialkylcarbamoylglycero-3-O-p-nitrophenyl alkylphosphonates, of different alkyl chain lengths. P. cepacia lipase was most rapidly, inactivated by Rc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl, octylphosphonate (Rc-trioctyl) with an inactivation half-time of 75 min, while that for the, Sc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl octyl-phosphonate, (Sc-trioctyl) compound was 530 min. X-ray structures were obtained of P., cepacia lipase after reaction with Rc-trioctyl to 0.29-nm resolution at pH, 4 and covalently modified with, Rc-(Rp,Sp)-1,2-dibutylcarbamoylglycero-3-O-p-nitrophenyl butyl-phosphonate, (Rc-tributyl) to 0.175-nm resolution ... [(full description)]
About this Structure
5LIP is a [Single protein] structure of sequence from [Burkholderia cepacia] with CA and OCP as [ligands]. Active as [Hydrolase], with EC number [3.1.1.3]. Structure known Active Sites: ACT and OXY. Full crystallographic information is available from [OCA].
Reference
Structural basis of the chiral selectivity of Pseudomonas cepacia lipase., Lang DA, Mannesse ML, de Haas GH, Verheij HM, Dijkstra BW, Eur J Biochem. 1998 Jun 1;254(2):333-40. PMID:9660188
Page seeded by OCA on Tue Oct 30 08:34:56 2007
