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2l4b

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[[Image:2l4b.png|left|200px]]
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==Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum. Seattle Structural Genomics Center for Infectious Disease target AnphA.01018.a==
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<StructureSection load='2l4b' size='340' side='right' caption='[[2l4b]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2l4b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anaplasma_phagocytophilum Anaplasma phagocytophilum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L4B FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">APH_0929 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=948 Anaplasma phagocytophilum])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l4b RCSB], [http://www.ebi.ac.uk/pdbsum/2l4b PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The botulinum neurotoxins (BoNTs) produced by different strains of the bacterium Clostridium botulinum are responsible for the disease botulism and include a group of immunologically distinct serotypes (A, B, E, and F) that are considered to be the most lethal natural proteins known for humans. Two BoNT serotypes, C and D, while rarely associated with human infection, are responsible for deadly botulism outbreaks afflicting animals. Also associated with animal infections is the BoNT C-D mosaic protein (BoNT/CD), a BoNT subtype that is essentially a hybrid of the BoNT/C ( approximately two-third) and BoNT/D ( approximately one-third) serotypes. While the amino acid sequence of the heavy chain receptor binding (HCR) domain of BoNT/CD (BoNT/CD-HCR) is very similar to the corresponding amino acid sequence of BoNT/D, BoNT/CD-HCR binds synaptosome membranes better than BoNT/D-HCR. To obtain structural insights for the different membrane binding properties, the crystal structure of BoNT/CD-HCR (S867-E1280) was determined at 1.56A resolution and compared to previously reported structures for BoNT/D-HCR. Overall, the BoNT/CD-HCR structure is similar to the two sub-domain organization observed for other BoNT HCRs: an N-terminal jellyroll barrel motif and a C-terminal beta-trefoil fold. Comparison of the structure of BoNT/CD-HCR with BoNT/D-HCR indicates that K1118 has a similar structural role as the equivalent residue, E1114, in BoNT/D-HCR, while K1136 has a structurally different role than the equivalent residue, G1132, in BoNT/D-HCR. Lysine-1118 forms a salt bridge with E1247 and may enhance membrane interactions by stabilizing the putative membrane binding loop (K1240-N1248). Lysine-1136 is observed on the surface of the protein. A sulfate ion bound to K1136 may mimic a natural interaction with the negatively changed phospholipid membrane surface. Liposome-binding experiments demonstrate that BoNT/CD-HCR binds phosphatidylethanolamine liposomes more tightly than BoNT/D-HCR.
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{{STRUCTURE_2l4b| PDB=2l4b | SCENE= }}
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Crystal structure of the receptor binding domain of the botulinum C-D mosaic neurotoxin reveals potential roles of lysines 1118 and 1136 in membrane interactions.,Zhang Y, Buchko GW, Qin L, Robinson H, Varnum SM Biochem Biophys Res Commun. 2011 Jan 7;404(1):407-12. Epub 2010 Dec 3. PMID:21130733<ref>PMID:21130733</ref>
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===Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum. Seattle Structural Genomics Center for Infectious Disease target AnphA.01018.a===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_21130733}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2l4b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anaplasma_phagocytophilum Anaplasma phagocytophilum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4B OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:021130733</ref><references group="xtra"/>
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[[Category: Anaplasma phagocytophilum]]
[[Category: Anaplasma phagocytophilum]]
[[Category: Buchko, G W.]]
[[Category: Buchko, G W.]]

Revision as of 11:25, 20 October 2014

Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum. Seattle Structural Genomics Center for Infectious Disease target AnphA.01018.a

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