This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1w9d

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1w9d.gif|left|200px]]<br /><applet load="1w9d" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1w9d.gif|left|200px]]
-
caption="1w9d, resolution 1.6&Aring;" />
+
 
-
'''S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE'''<br />
+
{{Structure
 +
|PDB= 1w9d |SIZE=350|CAPTION= <scene name='initialview01'>1w9d</scene>, resolution 1.6&Aring;
 +
|SITE= <scene name='pdbsite=AGB:Gol+Binding+Site+For+Chain+M'>AGB</scene>
 +
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SEH:S-BENZYL+PHENYLACETOTHIOHYDROXIMATE-O-SULFATE'>SEH</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Transferred_entry:_3.2.1.147 Transferred entry: 3.2.1.147], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1]
 +
|GENE=
 +
}}
 +
 
 +
'''S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1W9D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=SEH:'>SEH</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.2.1.147 Transferred entry: 3.2.1.147], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1] Known structural/functional Site: <scene name='pdbsite=AGB:Gol+Binding+Site+For+Chain+M'>AGB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9D OCA].
+
1W9D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9D OCA].
==Reference==
==Reference==
-
The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15889170 15889170]
+
The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15889170 15889170]
[[Category: Sinapis alba]]
[[Category: Sinapis alba]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 36: Line 45:
[[Category: thiohydroximate]]
[[Category: thiohydroximate]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:41:51 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:54:00 2008''

Revision as of 12:54, 20 March 2008

Image:1w9d.gif


PDB ID 1w9d

Drag the structure with the mouse to rotate
, resolution 1.6Å
Sites:
Ligands: , , , and
Activity: Transferred entry: 3.2.1.147, with EC number 3.2.3.1
Coordinates: save as pdb, mmCIF, xml



S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE


Overview

Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to hydrolyse glucosinolates, a unique family of molecules bearing an anomeric O-sulfated thiohydroximate function. Non-hydrolysable myrosinase inhibitors have been devised and studied for their biological interaction. Diverse modifications of the O-sulfate moiety did not result in a significant inhibitory effect, whereas replacing the D-glucopyrano residue by its carba-analogue allowed inhibition to take place. X-Ray experiments carried out after soaking allowed for the first time inclusion of a non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning of the aglycon part in its pocket is being used as a guide for the development of simplified and more potent inhibitors.

About this Structure

1W9D is a Single protein structure of sequence from Sinapis alba. Full crystallographic information is available from OCA.

Reference

The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:15889170

Page seeded by OCA on Thu Mar 20 14:54:00 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1w9d"
Personal tools