1wky

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[[Image:1wky.gif|left|200px]]<br /><applet load="1wky" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1wky.gif|left|200px]]
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caption="1wky, resolution 1.65&Aring;" />
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'''Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module'''<br />
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{{Structure
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|PDB= 1wky |SIZE=350|CAPTION= <scene name='initialview01'>1wky</scene>, resolution 1.65&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78]
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|GENE=
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}}
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'''Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1WKY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKY OCA].
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1WKY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKY OCA].
==Reference==
==Reference==
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Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15272186 15272186]
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Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15272186 15272186]
[[Category: Bacillus sp.]]
[[Category: Bacillus sp.]]
[[Category: Mannan endo-1,4-beta-mannosidase]]
[[Category: Mannan endo-1,4-beta-mannosidase]]
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[[Category: tim barrel]]
[[Category: tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:45:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:58:17 2008''

Revision as of 12:58, 20 March 2008

Image:1wky.gif


PDB ID 1wky

Drag the structure with the mouse to rotate
, resolution 1.65Å
Ligands: , and
Activity: Mannan endo-1,4-beta-mannosidase, with EC number 3.2.1.78
Coordinates: save as pdb, mmCIF, xml



Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module


Overview

An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence of the enzyme suggested that the enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method at 277 K. X-ray diffraction data were collected to 1.65 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(-1) and a solvent content of 45.6%. Molecular replacement for initial phasing was carried out using the three-dimensional structure of a mannanase from Thermomonospora fusca as a search model, which corresponds to the catalytic domain of the alkaline mannanase. It gave sufficient phases to build the unknown domain.

About this Structure

1WKY is a Protein complex structure of sequences from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:15272186

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