1wqs
From Proteopedia
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| - | [[Image:1wqs.gif|left|200px]] | + | [[Image:1wqs.gif|left|200px]] |
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| - | '''Crystal structure of Norovirus 3C-like protease''' | + | {{Structure |
| + | |PDB= 1wqs |SIZE=350|CAPTION= <scene name='initialview01'>1wqs</scene>, resolution 2.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene> and <scene name='pdbligand=TAR:D(-)-TARTARIC ACID'>TAR</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Norovirus 3C-like protease''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WQS is a [ | + | 1WQS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chiba_virus Chiba virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WQS OCA]. |
==Reference== | ==Reference== | ||
| - | A norovirus protease structure provides insights into active and substrate binding site integrity., Nakamura K, Someya Y, Kumasaka T, Ueno G, Yamamoto M, Sato T, Takeda N, Miyamura T, Tanaka N, J Virol. 2005 Nov;79(21):13685-93. PMID:[http:// | + | A norovirus protease structure provides insights into active and substrate binding site integrity., Nakamura K, Someya Y, Kumasaka T, Ueno G, Yamamoto M, Sato T, Takeda N, Miyamura T, Tanaka N, J Virol. 2005 Nov;79(21):13685-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16227288 16227288] |
[[Category: Chiba virus]] | [[Category: Chiba virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxyanion hole]] | [[Category: oxyanion hole]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:00:27 2008'' |
Revision as of 13:00, 20 March 2008
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| , resolution 2.80Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Norovirus 3C-like protease
Overview
Norovirus 3C-like proteases are crucial to proteolytic processing of norovirus polyproteins. We determined the crystal structure of the 3C-like protease from Chiba virus, a norovirus, at 2.8-A resolution. An active site including Cys139 and His30 is present, as is a hydrogen bond network that stabilizes the active site conformation. In the oxyanion hole backbone, a structural difference was observed probably upon substrate binding. A peptide substrate/enzyme model shows that several interactions between the two components are critical for substrate binding and that the S1 and S2 sites appropriately accommodate the substrate P1 and P2 residues, respectively. Knowledge of the structure and a previous mutagenesis study allow us to correlate proteolysis and structure.
About this Structure
1WQS is a Single protein structure of sequence from Chiba virus. Full crystallographic information is available from OCA.
Reference
A norovirus protease structure provides insights into active and substrate binding site integrity., Nakamura K, Someya Y, Kumasaka T, Ueno G, Yamamoto M, Sato T, Takeda N, Miyamura T, Tanaka N, J Virol. 2005 Nov;79(21):13685-93. PMID:16227288
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