1h1l
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 1H1L is a | + | 1H1L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with MG, CL, CIT, CFM and CLF as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1] Structure known Active Site: CT1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H1L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:01:54 2007'' |
Revision as of 10:56, 5 November 2007
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NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT
Overview
The x-ray crystal structure of NifV(-) Klebsiella pneumoniae nitrogenase, MoFe protein (NifV(-) Kp1) has been determined and refined to a resolution, of 1.9 A. This is the first structure for a nitrogenase MoFe protein with, an altered cofactor. Moreover, it is the first direct evidence that the, organic acid citrate is not just present, but replaces homocitrate as a, ligand to the molybdenum atom of the iron molybdenum cofactor (FeMoco)., Subsequent refinement of the structure revealed that the citrate was, present at reduced occupancy.
About this Structure
1H1L is a Protein complex structure of sequences from Klebsiella pneumoniae with MG, CL, CIT, CFM and CLF as ligands. Active as Nitrogenase, with EC number 1.18.6.1 Structure known Active Site: CT1. Full crystallographic information is available from OCA.
Reference
Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco)., Mayer SM, Gormal CA, Smith BE, Lawson DM, J Biol Chem. 2002 Sep 20;277(38):35263-6. Epub 2002 Jul 19. PMID:12133839
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