2jlt

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[[Image:2jlt.png|left|200px]]
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==CRYSTAL STRUCTURE OF AN RNA KISSING COMPLEX==
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<StructureSection load='2jlt' size='340' side='right' caption='[[2jlt]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jlt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JLT FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5BU:5-BROMO-URIDINE-5-MONOPHOSPHATE'>5BU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jlt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jlt RCSB], [http://www.ebi.ac.uk/pdbsum/2jlt PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In HIV-1, trans-activation of transcription of the viral genome is regulated by an imperfect hairpin, the trans-activating responsive (TAR) RNA element, located at the 5' untranslated end of all viral transcripts. TAR acts as a binding site for viral and cellular proteins. In an attempt to identify RNA ligands that would interfere with the virus life-cycle by interacting with TAR, an in vitro selection was previously carried out. RNA hairpins that formed kissing-loop dimers with TAR were selected [Duconge F. and Toulme JJ (1999) RNA, 5:1605-1614]. We describe here the crystal structure of TAR bound to a high-affinity RNA aptamer. The two hairpins form a kissing complex and interact through six Watson-Crick base pairs. The complex adopts an overall conformation with an inter-helix angle of 28.1 degrees , thus contrasting with previously reported solution and modelling studies. Structural analysis reveals that inter-backbone hydrogen bonds between ribose 2' hydroxyl and phosphate oxygens at the stem-loop junctions can be formed. Thermal denaturation and surface plasmon resonance experiments with chemically modified 2'-O-methyl incorporated into both hairpins at key positions, clearly demonstrate the involvement of this intermolecular network of hydrogen bonds in complex stability.
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{{STRUCTURE_2jlt| PDB=2jlt | SCENE= }}
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Exploring TAR-RNA aptamer loop-loop interaction by X-ray crystallography, UV spectroscopy and surface plasmon resonance.,Lebars I, Legrand P, Aime A, Pinaud N, Fribourg S, Di Primo C Nucleic Acids Res. 2008 Dec;36(22):7146-56. Epub 2008 Nov 7. PMID:18996893<ref>PMID:18996893</ref>
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===CRYSTAL STRUCTURE OF AN RNA KISSING COMPLEX===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18996893}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2jlt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JLT OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:018996893</ref><references group="xtra"/>
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[[Category: Diprimo, C.]]
[[Category: Diprimo, C.]]
[[Category: Fribourg, S.]]
[[Category: Fribourg, S.]]
[[Category: Kissing complex]]
[[Category: Kissing complex]]
[[Category: Rna]]
[[Category: Rna]]

Revision as of 15:29, 12 October 2014

CRYSTAL STRUCTURE OF AN RNA KISSING COMPLEX

2jlt, resolution 2.90Å

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