1x18
From Proteopedia
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| - | [[Image:1x18.gif|left|200px]] | + | [[Image:1x18.gif|left|200px]] |
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| - | '''Contact sites of ERA GTPase on the THERMUS THERMOPHILUS 30S SUBUNIT''' | + | {{Structure |
| + | |PDB= 1x18 |SIZE=350|CAPTION= <scene name='initialview01'>1x18</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Contact sites of ERA GTPase on the THERMUS THERMOPHILUS 30S SUBUNIT''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1X18 is a [ | + | 1X18 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X18 OCA]. |
==Reference== | ==Reference== | ||
| - | Interaction of Era with the 30S ribosomal subunit implications for 30S subunit assembly., Sharma MR, Barat C, Wilson DN, Booth TM, Kawazoe M, Hori-Takemoto C, Shirouzu M, Yokoyama S, Fucini P, Agrawal RK, Mol Cell. 2005 Apr 29;18(3):319-29. PMID:[http:// | + | Interaction of Era with the 30S ribosomal subunit implications for 30S subunit assembly., Sharma MR, Barat C, Wilson DN, Booth TM, Kawazoe M, Hori-Takemoto C, Shirouzu M, Yokoyama S, Fucini P, Agrawal RK, Mol Cell. 2005 Apr 29;18(3):319-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15866174 15866174] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: contact sites of era protein on the 30s ribosomal subunit]] | [[Category: contact sites of era protein on the 30s ribosomal subunit]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:03:55 2008'' |
Revision as of 13:03, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Contact sites of ERA GTPase on the THERMUS THERMOPHILUS 30S SUBUNIT
Overview
Era (E. coliRas-like protein) is a highly conserved and essential GTPase in bacteria. It binds to the 16S ribosomal RNA (rRNA) of the small (30S) ribosomal subunit, and its depletion leads to accumulation of an unprocessed precursor of the 16S rRNA. We have obtained a three-dimensional cryo-electron microscopic map of the Thermus thermophilus 30S-Era complex. Era binds in the cleft between the head and platform of the 30S subunit and locks the subunit in a conformation that is not favorable for association with the large (50S) ribosomal subunit. The RNA binding KH motif present within the C-terminal domain of Era interacts with the conserved nucleotides in the 3' region of the 16S rRNA. Furthermore, Era makes contact with several assembly elements of the 30S subunit. These observations suggest a direct involvement of Era in the assembly and maturation of the 30S subunit.
About this Structure
1X18 is a Protein complex structure of sequences from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Interaction of Era with the 30S ribosomal subunit implications for 30S subunit assembly., Sharma MR, Barat C, Wilson DN, Booth TM, Kawazoe M, Hori-Takemoto C, Shirouzu M, Yokoyama S, Fucini P, Agrawal RK, Mol Cell. 2005 Apr 29;18(3):319-29. PMID:15866174
Page seeded by OCA on Thu Mar 20 15:03:55 2008
