1x2x
From Proteopedia
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| - | [[Image:1x2x.gif|left|200px]] | + | [[Image:1x2x.gif|left|200px]] |
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| - | '''NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings''' | + | {{Structure |
| + | |PDB= 1x2x |SIZE=350|CAPTION= <scene name='initialview01'>1x2x</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1X2X is a [ | + | 1X2X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X2X OCA]. |
==Reference== | ==Reference== | ||
| - | NMR assessment of the global shape of a non-labelled DNA dodecamer containing a tandem of G-T mismatches., Alvarez-Salgado F, Desvaux H, Boulard Y, Magn Reson Chem. 2006 Dec;44(12):1081-9. PMID:[http:// | + | NMR assessment of the global shape of a non-labelled DNA dodecamer containing a tandem of G-T mismatches., Alvarez-Salgado F, Desvaux H, Boulard Y, Magn Reson Chem. 2006 Dec;44(12):1081-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16972306 16972306] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Alvarez-Salgado, F.]] | [[Category: Alvarez-Salgado, F.]] | ||
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[[Category: nmr]] | [[Category: nmr]] | ||
[[Category: rdc]] | [[Category: rdc]] | ||
| - | [[Category: tandem gt | + | [[Category: tandem gt mismatch]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:04:30 2008'' |
Revision as of 13:04, 20 March 2008
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NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings
Overview
We have carried out a solution study of two non-labelled self-complementary DNA dodecamers d(GACTGTACAGTC)2 and d(GACTGTGCAGTC)2 by NMR, the second sequence composed of two G-T mismatches. Structures were determined using distances extracted from NOE effects alone or using both NOE and RDC constraints, measured in three different liquid crystalline media. We ensured that our data on the influence of the mesogen on the DNA structures, and the way in which the RDCs were incorporated as constraints in the protocol refinement, were consistent. We also tested the influence of different sets of RDCs and the best means of optimizing the calculation of D(a) and R. Resolution and accuracy of the ten best energy final structures were compared. The addition of a small set of RDC constraints significantly improves the final determined structures. We took advantage of the specificity of the RDC, i.e. it contains orientational information, and explored the global shape of the DNA duplexes; it was found that the duplexes do not have a large curvature. For the G-T base pair, we observed, in this particular sequence (tandem of G-T mismatches), a new pattern of base pairing, which involved the formation of a bifurcated hydrogen bond.
About this Structure
1X2X is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR assessment of the global shape of a non-labelled DNA dodecamer containing a tandem of G-T mismatches., Alvarez-Salgado F, Desvaux H, Boulard Y, Magn Reson Chem. 2006 Dec;44(12):1081-9. PMID:16972306
Page seeded by OCA on Thu Mar 20 15:04:30 2008
