3dbo
From Proteopedia
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| - | [[ | + | ==Crystal structure of a member of the VapBC family of toxin-antitoxin systems, VapBC-5, from Mycobacterium tuberculosis== |
| + | <StructureSection load='3dbo' size='340' side='right' caption='[[3dbo]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3dbo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DBO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DBO FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv0626, MT0654 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis]), Rv0627 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dbo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dbo RCSB], [http://www.ebi.ac.uk/pdbsum/3dbo PDBsum], [http://www.topsan.org/Proteins/ISFI/3dbo TOPSAN]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/3dbo_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In prokaryotes, cognate toxin-antitoxin pairs have long been known, but no three-dimensional structure has been available for any given complex from Mycobacterium tuberculosis. Here we report the crystal structure and activity of a member of the VapBC family of complexes from M. tuberculosis. The toxin VapC-5 is a compact, 150 residues, two domain alpha/beta protein. Bent around the toxin is the VapB-5 antitoxin, a 33-residue alpha-helix. Assays suggest that the toxin is an Mg-enabled endoribonuclease, inhibited by the antitoxin. The lack of DNase activity is consistent with earlier suggestions that the complex represses its own operon. Furthermore, analysis of the interactions in the binding of the antitoxin to the toxin suggest that exquisite control is required to protect the bacteria cell from toxic VapC-5. | ||
| - | + | Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis.,Miallau L, Faller M, Chiang J, Arbing M, Guo F, Cascio D, Eisenberg D J Biol Chem. 2009 Jan 2;284(1):276-83. Epub 2008 Oct 24. PMID:18952600<ref>PMID:18952600</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
| - | [[Category: Cascio, D | + | [[Category: Cascio, D]] |
| - | [[Category: Eisenberg, D | + | [[Category: Eisenberg, D]] |
| - | [[Category: ISFI, Integrated Center for Structure and Function Innovation | + | [[Category: ISFI, Integrated Center for Structure and Function Innovation]] |
| - | [[Category: Miallau, L | + | [[Category: Miallau, L]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
[[Category: Integrated center for structure and function innovation]] | [[Category: Integrated center for structure and function innovation]] | ||
[[Category: Isfi]] | [[Category: Isfi]] | ||
| - | [[Category: | + | [[Category: PSI, Protein structure initiative]] |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
[[Category: Tbsgc]] | [[Category: Tbsgc]] | ||
[[Category: Toxin antitoxin complex]] | [[Category: Toxin antitoxin complex]] | ||
[[Category: Toxin-antitoxin complex]] | [[Category: Toxin-antitoxin complex]] | ||
[[Category: Vapbc]] | [[Category: Vapbc]] | ||
Revision as of 08:03, 12 November 2014
Crystal structure of a member of the VapBC family of toxin-antitoxin systems, VapBC-5, from Mycobacterium tuberculosis
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Categories: Mycobacterium tuberculosis | Cascio, D | Eisenberg, D | ISFI, Integrated Center for Structure and Function Innovation | Miallau, L | Structural genomic | Integrated center for structure and function innovation | Isfi | PSI, Protein structure initiative | Tbsgc | Toxin antitoxin complex | Toxin-antitoxin complex | Vapbc
