3d6l

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[[Image:3d6l.png|left|200px]]
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==Crystal structure of Cj0915, a hexameric hotdog fold thioesterase of Campylobacter jejuni==
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<StructureSection load='3d6l' size='340' side='right' caption='[[3d6l]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3d6l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3D6L FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cj0915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 Campylobacter jejuni])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d6l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3d6l RCSB], [http://www.ebi.ac.uk/pdbsum/3d6l PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d6/3d6l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Acyl-coenzyme A (CoA) thioesterases are a large family of enzymes that hydrolyze acyl-CoA esters to the free fatty acid and CoA and thereby regulate essential cellular functions such as lipid metabolism, membrane synthesis, signal transduction, and gene transcription. To better understand the virulence mechanisms of Campylobacter jejuni, and its possible link to membrane lipid biosynthesis, we have investigated C. jejuni thioesterases, annotated as putative proteins. While little is known about fatty acid biosynthesis and regulation in C. jejuni, remarkable differences in the genome and its organization from Escherichia coli, the paradigm system, raise questions as to the functions of these putative proteins. Here we present the crystal structure and biochemical analysis of Cj0915, defining the first functional thioesterase from C. jejuni. The structure of Cj0915 reveals the hot dog fold with an YciA-type hexameric assembly. Enzymatic assays performed with the purified protein show that Cj0915 is an efficient thioesterase with a broad specificity toward acyl-CoA substrates. This study provides a framework for investigation on roles of the Cj0915 thioesterase in virulence, and functional activities associated with the Cj0915 thioesterase in vivo.
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{{STRUCTURE_3d6l| PDB=3d6l | SCENE= }}
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Structure and function of a Campylobacter jejuni thioesterase Cj0915, a hexameric hot dog fold enzyme.,Yokoyama T, Choi KJ, Bosch AM, Yeo HJ Biochim Biophys Acta. 2009 Jul;1794(7):1073-81. Epub 2009 Mar 17. PMID:19303060<ref>PMID:19303060</ref>
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===Crystal structure of Cj0915, a hexameric hotdog fold thioesterase of Campylobacter jejuni===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19303060}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3d6l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D6L OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019303060</ref><references group="xtra"/>
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[[Category: Campylobacter jejuni]]
[[Category: Campylobacter jejuni]]
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[[Category: Yeo, H J.]]
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[[Category: Yeo, H J]]
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[[Category: Yokoyama, T.]]
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[[Category: Yokoyama, T]]
[[Category: Acyl-coa]]
[[Category: Acyl-coa]]
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[[Category: Campylobacter jejuni]]
 
[[Category: Hot dog fold]]
[[Category: Hot dog fold]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Thioesterase]]
[[Category: Thioesterase]]

Revision as of 08:04, 12 November 2014

Crystal structure of Cj0915, a hexameric hotdog fold thioesterase of Campylobacter jejuni

3d6l, resolution 2.59Å

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