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1xcb
From Proteopedia
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| - | [[Image:1xcb.jpg|left|200px]] | + | [[Image:1xcb.jpg|left|200px]] |
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| - | '''X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus''' | + | {{Structure |
| + | |PDB= 1xcb |SIZE=350|CAPTION= <scene name='initialview01'>1xcb</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= rex ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus]) | ||
| + | }} | ||
| + | |||
| + | '''X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XCB is a [ | + | 1XCB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. This structure supersedes the now removed PDB entry 1R72. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCB OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing., Sickmier EA, Brekasis D, Paranawithana S, Bonanno JB, Paget MS, Burley SK, Kielkopf CL, Structure. 2005 Jan;13(1):43-54. PMID:[http:// | + | X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing., Sickmier EA, Brekasis D, Paranawithana S, Bonanno JB, Paget MS, Burley SK, Kielkopf CL, Structure. 2005 Jan;13(1):43-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15642260 15642260] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus aquaticus]] | [[Category: Thermus aquaticus]] | ||
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[[Category: rex]] | [[Category: rex]] | ||
[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: thermus aquaticus]] | [[Category: thermus aquaticus]] | ||
[[Category: winged helix]] | [[Category: winged helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:07:51 2008'' |
Revision as of 13:07, 20 March 2008
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| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | rex (Thermus aquaticus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus
Overview
The redox-sensing repressor Rex regulates transcription of respiratory genes in response to the intra cellular NADH/NAD(+) redox poise. As a step toward elucidating the molecular mechanism of NADH/NAD(+) sensing, the X-ray structure of Thermus aquaticus Rex (T-Rex) bound to effector NADH has been determined at 2.9 A resolution. The fold of the C-terminal domain of T-Rex is characteristic of NAD(H)-dependent enzymes, whereas the N-terminal domain is similar to a winged helix DNA binding motif. T-Rex dimerization is primarily mediated by "domain-swapped" alpha helices. Each NADH molecule binds to the C-terminal domain near the dimer interface. In contrast to NAD(H)-dependent enzymes, the nicotinamide is deeply buried within a hydrophobic pocket that appears to preclude substrate entry. We show that T-Rex binds to the Rex operator, and NADH but not NAD(+) inhibits T-Rex/DNA binding activity. A mechanism for redox sensing by Rex family members is proposed by analogy with domain closure of NAD(H)-dependent enzymes.
About this Structure
1XCB is a Single protein structure of sequence from Thermus aquaticus. This structure supersedes the now removed PDB entry 1R72. Full crystallographic information is available from OCA.
Reference
X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing., Sickmier EA, Brekasis D, Paranawithana S, Bonanno JB, Paget MS, Burley SK, Kielkopf CL, Structure. 2005 Jan;13(1):43-54. PMID:15642260
Page seeded by OCA on Thu Mar 20 15:07:51 2008
Categories: Single protein | Thermus aquaticus | Bonanno, J B. | Brekasis, D. | Burley, S K. | Kielkopf, C L. | NYSGXRC, New York Structural GenomiX Research Consortium. | Paget, M S. | Paranawithana, S. | Sickmier, E A. | CA | NAD | Nad | New york structural genomix research consortium | Nicotinamide adenine dinucleotide | Nysgxrc | Protein structure initiative | Psi | Redox-sensing | Rex | Rossmann fold | Structural genomic | Winged helix
