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2wsp

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[[Image:2wsp.png|left|200px]]
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==THERMOTOGA MARITIMA ALPHA-L-FUCOSYNTHASE, TMD224G, IN COMPLEX WITH ALPHA-L-FUC-(1-2)-BETA-L-FUC-N3==
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<StructureSection load='2wsp' size='340' side='right' caption='[[2wsp]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wsp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WSP FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUY:BETA-L-FUCOSYL-AZIDE'>FUY</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1odu|1odu]], [[1hl9|1hl9]], [[1hl8|1hl8]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-L-fucosidase Alpha-L-fucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.51 3.2.1.51] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wsp RCSB], [http://www.ebi.ac.uk/pdbsum/2wsp PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Fucose-containing oligosaccharides play a central role in physio-pathological events, and fucosylated oligosaccharides have interesting potential applications in biomedicine. No methods for the large-scale production of oligosaccharides are currently available, but the chemo-enzymatic approach is very promising. Glycosynthases, mutated glycosidases that synthesize oligosaccharides in high yields, have been demonstrated to be an interesting alternative. However, examples of glycosynthases available so far are restricted to a limited number of glycosidases families and to only one retaining alpha-glycosynthase. We show here that new mutants of two alpha-L-fucosidases are efficient alpha-L-fucosynthases. The approach shown utilized beta-L-fucopyranosyl azide as donor substrate leading to transglycosylation yields up to 91%. This is the first method exploiting a beta-glycosyl azide donor for alpha-glycosynthases; its applicability to the glycosynthetic methodology in a wider perspective is presented.
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{{STRUCTURE_2wsp| PDB=2wsp | SCENE= }}
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beta-Glycosyl azides as substrates for alpha-glycosynthases: preparation of efficient alpha-L-fucosynthases.,Cobucci-Ponzano B, Conte F, Bedini E, Corsaro MM, Parrilli M, Sulzenbacher G, Lipski A, Dal Piaz F, Lepore L, Rossi M, Moracci M Chem Biol. 2009 Oct 30;16(10):1097-108. PMID:19875083<ref>PMID:19875083</ref>
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===THERMOTOGA MARITIMA ALPHA-L-FUCOSYNTHASE, TMD224G, IN COMPLEX WITH ALPHA-L-FUC-(1-2)-BETA-L-FUC-N3===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19875083}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2wsp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSP OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019875083</ref><references group="xtra"/>
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[[Category: Alpha-L-fucosidase]]
[[Category: Alpha-L-fucosidase]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]

Revision as of 02:20, 1 October 2014

THERMOTOGA MARITIMA ALPHA-L-FUCOSYNTHASE, TMD224G, IN COMPLEX WITH ALPHA-L-FUC-(1-2)-BETA-L-FUC-N3

2wsp, resolution 2.65Å

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