1xu6
From Proteopedia
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| - | [[Image:1xu6.gif|left|200px]] | + | [[Image:1xu6.gif|left|200px]] |
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| - | '''Structure of the C-terminal domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2''' | + | {{Structure |
| + | |PDB= 1xu6 |SIZE=350|CAPTION= <scene name='initialview01'>1xu6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Structure of the C-terminal domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XU6 is a [ | + | 1XU6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XU6 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the C-terminal domain from Trypanosoma brucei variant surface glycoprotein MITat1.2., Chattopadhyay A, Jones NG, Nietlispach D, Nielsen PR, Voorheis HP, Mott HR, Carrington M, J Biol Chem. 2005 Feb 25;280(8):7228-35. Epub 2004 Nov 22. PMID:[http:// | + | Structure of the C-terminal domain from Trypanosoma brucei variant surface glycoprotein MITat1.2., Chattopadhyay A, Jones NG, Nietlispach D, Nielsen PR, Voorheis HP, Mott HR, Carrington M, J Biol Chem. 2005 Feb 25;280(8):7228-35. Epub 2004 Nov 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15557330 15557330] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Trypanosoma brucei brucei]] | [[Category: Trypanosoma brucei brucei]] | ||
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[[Category: cysteine knot]] | [[Category: cysteine knot]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:14:33 2008'' |
Revision as of 13:14, 20 March 2008
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Structure of the C-terminal domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2
Overview
The variant surface glycoprotein (VSG) of African trypanosomes has a structural role in protecting other cell surface proteins from effector molecules of the mammalian immune system and also undergoes antigenic variation necessary for a persistent infection in a host. Here we have reported the solution structure of a VSG type 2 C-terminal domain from MITat1.2, completing the first structure of both domains of a VSG. The isolated C-terminal domain is a monomer in solution and forms a novel fold, which commences with a short alpha-helix followed by a single turn of 3(10)-helix and connected by a short loop to a small anti-parallel beta-sheet and then a longer alpha-helix at the C terminus. This compact domain is flanked by two unstructured regions. The structured part of the domain contains 42 residues, and the core comprises 2 disulfide bonds and 2 hydrophobic residues. These cysteines and hydrophobic residues are conserved in other VSGs, and we have modeled the structures of two further VSG C-terminal domains using the structure of MITat1.2. The models suggest that the overall structure of the core is conserved in the different VSGs but that the C-terminal alpha-helix is of variable length and depends on the presence of charged residues. The results provided evidence for a conserved tertiary structure for all the type 2 VSG C-terminal domains, indicated that VSG dimers form through interactions between N-terminal domains, and showed that the selection pressure for sequence variation within a conserved tertiary structure acts on the whole of the VSG molecule.
About this Structure
1XU6 is a Single protein structure of sequence from Trypanosoma brucei brucei. Full crystallographic information is available from OCA.
Reference
Structure of the C-terminal domain from Trypanosoma brucei variant surface glycoprotein MITat1.2., Chattopadhyay A, Jones NG, Nietlispach D, Nielsen PR, Voorheis HP, Mott HR, Carrington M, J Biol Chem. 2005 Feb 25;280(8):7228-35. Epub 2004 Nov 22. PMID:15557330
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