1xwt
From Proteopedia
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| - | [[Image:1xwt.gif|left|200px]] | + | [[Image:1xwt.gif|left|200px]] |
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| - | '''Structure Of A Cold-Adapted Family 8 Xylanase''' | + | {{Structure |
| + | |PDB= 1xwt |SIZE=350|CAPTION= <scene name='initialview01'>1xwt</scene>, resolution 1.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure Of A Cold-Adapted Family 8 Xylanase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XWT is a [ | + | 1XWT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudoalteromonas_haloplanktis Pseudoalteromonas haloplanktis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XWT OCA]. |
==Reference== | ==Reference== | ||
| - | Study of the active site residues of a glycoside hydrolase family 8 xylanase., Collins T, De Vos D, Hoyoux A, Savvides SN, Gerday C, Van Beeumen J, Feller G, J Mol Biol. 2005 Nov 25;354(2):425-35. Epub 2005 Oct 10. PMID:[http:// | + | Study of the active site residues of a glycoside hydrolase family 8 xylanase., Collins T, De Vos D, Hoyoux A, Savvides SN, Gerday C, Van Beeumen J, Feller G, J Mol Biol. 2005 Nov 25;354(2):425-35. Epub 2005 Oct 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16246370 16246370] |
[[Category: Endo-1,4-beta-xylanase]] | [[Category: Endo-1,4-beta-xylanase]] | ||
[[Category: Pseudoalteromonas haloplanktis]] | [[Category: Pseudoalteromonas haloplanktis]] | ||
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[[Category: xylan degradation]] | [[Category: xylan degradation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:15:30 2008'' |
Revision as of 13:15, 20 March 2008
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| , resolution 1.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Endo-1,4-beta-xylanase, with EC number 3.2.1.8 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure Of A Cold-Adapted Family 8 Xylanase
Overview
Site-directed mutagenesis and a comparative characterisation of the kinetic parameters, pH dependency of activity and thermal stability of mutant and wild-type enzymes have been used in association with crystallographic analysis to delineate the functions of several active site residues in a novel glycoside hydrolase family 8 xylanase. Each of the residues investigated plays an essential role in this enzyme: E78 as the general acid, D281 as the general base and in orientating the nucleophilic water molecule, Y203 in maintaining the position of the nucleophilic water molecule and in structural integrity and D144 in sugar ring distortion and transition state stabilization. Interestingly, although crystal structure analyses and the pH-activity profiles clearly identify the functions of E78 and D281, substitution of these residues with their amide derivatives results in only a 250-fold and 700-fold reduction in their apparent k(cat) values, respectively. This, in addition to the observation that the proposed general base is not conserved in all glycoside hydrolase family 8 enzymes, indicates that the mechanistic architecture in this family of inverting enzymes is more complex than is conventionally believed and points to a diversity in the identity of the mechanistically important residues as well as in the arrangement of the intricate microenvironment of the active site among members of this family.
About this Structure
1XWT is a Single protein structure of sequence from Pseudoalteromonas haloplanktis. Full crystallographic information is available from OCA.
Reference
Study of the active site residues of a glycoside hydrolase family 8 xylanase., Collins T, De Vos D, Hoyoux A, Savvides SN, Gerday C, Van Beeumen J, Feller G, J Mol Biol. 2005 Nov 25;354(2):425-35. Epub 2005 Oct 10. PMID:16246370
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