1y1y
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1y1y.gif|left|200px]] | + | [[Image:1y1y.gif|left|200px]] |
| - | + | ||
| - | '''RNA Polymerase II-TFIIS-DNA/RNA complex''' | + | {{Structure |
| + | |PDB= 1y1y |SIZE=350|CAPTION= <scene name='initialview01'>1y1y</scene>, resolution 4.Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | ||
| + | |GENE= DST1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''RNA Polymerase II-TFIIS-DNA/RNA complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1Y1Y is a [ | + | 1Y1Y is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y1Y OCA]. |
==Reference== | ==Reference== | ||
| - | Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS., Kettenberger H, Armache KJ, Cramer P, Mol Cell. 2004 Dec 22;16(6):955-65. PMID:[http:// | + | Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS., Kettenberger H, Armache KJ, Cramer P, Mol Cell. 2004 Dec 22;16(6):955-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15610738 15610738] |
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 17: | Line 26: | ||
[[Category: Cramer, P.]] | [[Category: Cramer, P.]] | ||
[[Category: Kettenberger, H.]] | [[Category: Kettenberger, H.]] | ||
| - | [[Category: nucleic | + | [[Category: nucleic acid]] |
[[Category: readthrough]] | [[Category: readthrough]] | ||
[[Category: rna polymerase ii]] | [[Category: rna polymerase ii]] | ||
| - | [[Category: | + | [[Category: tfii]] |
[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:17:27 2008'' |
Revision as of 13:17, 20 March 2008
| |||||||
| , resolution 4.Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | DST1 (Saccharomyces cerevisiae) | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA Polymerase II-TFIIS-DNA/RNA complex
Overview
The crystal structure of the complete 12 subunit RNA polymerase (pol) II bound to a transcription bubble and product RNA reveals incoming template and nontemplate DNA, a seven base pair DNA/RNA hybrid, and three nucleotides each of separating DNA and RNA. The complex adopts the posttranslocation state and accommodates a cocrystallized nucleoside triphosphate (NTP) substrate. The NTP binds in the active site pore at a position to interact with a DNA template base. Residues surrounding the NTP are conserved in all cellular RNA polymerases, suggesting a universal mechanism of NTP selection and incorporation. DNA-DNA and DNA-RNA strand separation may be explained by pol II-induced duplex distortions. Four protein loops partition the active center cleft, contribute to embedding the hybrid, prevent strand reassociation, and create an RNA exit tunnel. Binding of the elongation factor TFIIS realigns RNA in the active center, possibly converting the elongation complex to an alternative state less prone to stalling.
About this Structure
1Y1Y is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS., Kettenberger H, Armache KJ, Cramer P, Mol Cell. 2004 Dec 22;16(6):955-65. PMID:15610738
Page seeded by OCA on Thu Mar 20 15:17:27 2008
