2wkn
From Proteopedia
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| - | [[ | + | ==GAMMA LACTAMASE FROM DELFTIA ACIDOVORANS== |
| + | <StructureSection load='2wkn' size='340' side='right' caption='[[2wkn]], [[Resolution|resolution]] 2.08Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2wkn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKN FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formamidase Formamidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.49 3.5.1.49] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wkn RCSB], [http://www.ebi.ac.uk/pdbsum/2wkn PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wkn_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | An enzyme from Comomonas acidovorans has been isolated that is specific for the stereospecific hydrolysis of (+)gamma-lactam. This so-called (+)gamma-lactamase has important applications in biotransformation reactions. The enzyme has been crystallized by vapour-phase diffusion using polyethylene glycol 4000 as a precipitant. Addition of a detergent, beta-octylglucoside, was found to be essential for obtaining diffraction-quality crystals. The crystals grow in the space group P1, with unit-cell parameters a = 63.0, b = 93.2, c = 152.4 A, alpha = 104.3, beta = 92.6, gamma = 108.5 degrees, and diffract to 2 A resolution using synchrotron radiation. Native data from these crystals have been collected to 2.4 A. | ||
| - | + | Crystallization and preliminary X-ray analysis of a gamma-lactamase.,Gonsalvez IS, Isupov MN, Littlechild JA Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):284-6. PMID:11173481<ref>PMID:11173481</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Delftia acidovorans]] | [[Category: Delftia acidovorans]] | ||
[[Category: Formamidase]] | [[Category: Formamidase]] | ||
Revision as of 11:22, 20 October 2014
GAMMA LACTAMASE FROM DELFTIA ACIDOVORANS
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