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1yc6
From Proteopedia
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| - | [[Image:1yc6.gif|left|200px]] | + | [[Image:1yc6.gif|left|200px]] |
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| - | '''Crystallographic Structure of the T=1 Particle of Brome Mosaic Virus''' | + | {{Structure |
| + | |PDB= 1yc6 |SIZE=350|CAPTION= <scene name='initialview01'>1yc6</scene>, resolution 2.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystallographic Structure of the T=1 Particle of Brome Mosaic Virus''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YC6 is a [ | + | 1YC6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Brome_mosaic_virus Brome mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YC6 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallographic structure of the T=1 particle of brome mosaic virus., Larson SB, Lucas RW, McPherson A, J Mol Biol. 2005 Feb 25;346(3):815-31. Epub 2005 Jan 12. PMID:[http:// | + | Crystallographic structure of the T=1 particle of brome mosaic virus., Larson SB, Lucas RW, McPherson A, J Mol Biol. 2005 Feb 25;346(3):815-31. Epub 2005 Jan 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713465 15713465] |
[[Category: Brome mosaic virus]] | [[Category: Brome mosaic virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: symmetry]] | [[Category: symmetry]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:20:59 2008'' |
Revision as of 13:21, 20 March 2008
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Crystallographic Structure of the T=1 Particle of Brome Mosaic Virus
Overview
T=1 icosahedral particles of amino terminally truncated brome mosaic virus (BMV) protein were created by treatment of the wild-type T=3 virus with 1M CaCl2 and crystallized from sodium malonate. Diffraction data were collected from frozen crystals to beyond 2.9 A resolution and the structure determined by molecular replacement and phase extension. The particles are composed of pentameric capsomeres from the wild-type virions which have reoriented with respect to the original particle pentameric axes by rotations of 37 degrees , and formed tenuous interactions with one another, principally through conformationally altered C-terminal polypeptides. Otherwise, the pentamers are virtually superimposable upon those of the original T=3 BMV particles. The T=1 particles, in the crystals, are not perfect icosahedra, but deviate slightly from exact symmetry, possibly due to packing interactions. This suggests that the T=1 particles are deformable, which is consistent with the loose arrangement of pentamers and latticework of holes that penetrate the surface. Atomic force microscopy showed that the T=3 to T=1 transition could occur by shedding of hexameric capsomeres and restructuring of remaining pentamers accompanied by direct condensation. Knowledge of the structures of the BMV wild-type and T=1 particles now permit us to propose a tentative model for that process. A comparison of the BMV T=1 particles was made with the reassembled T=1 particles produced from the coat protein of trypsin treated alfalfa mosaic virus (AlMV), another bromovirus. There is little resemblance between the two particles. The BMV particle, with a maximum diameter of 195 A, is made from distinctive pentameric capsomeres with large holes along the 3-fold axis, while the AlMV particle, of approximate maximum diameter 220 A, has subunits closely packed around the 3-fold axis, large holes along the 5-fold axis, and few contacts within pentamers. In both particles crucial linkages are made about icosahedral dyads.
About this Structure
1YC6 is a Single protein structure of sequence from Brome mosaic virus. Full crystallographic information is available from OCA.
Reference
Crystallographic structure of the T=1 particle of brome mosaic virus., Larson SB, Lucas RW, McPherson A, J Mol Biol. 2005 Feb 25;346(3):815-31. Epub 2005 Jan 12. PMID:15713465
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