1yfe
From Proteopedia
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| - | [[Image:1yfe.gif|left|200px]] | + | [[Image:1yfe.gif|left|200px]] |
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| - | '''Crystal structure of apo fumarase C from Escherichia coli''' | + | {{Structure |
| + | |PDB= 1yfe |SIZE=350|CAPTION= <scene name='initialview01'>1yfe</scene>, resolution 2.19Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of apo fumarase C from Escherichia coli''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YFE is a [ | + | 1YFE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YFE OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of free fumarase C from Escherichia coli., Weaver T, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1395-401. Epub, 2005 Sep 28. PMID:[http:// | + | Structure of free fumarase C from Escherichia coli., Weaver T, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1395-401. Epub, 2005 Sep 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16204892 16204892] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Fumarate hydratase]] | [[Category: Fumarate hydratase]] | ||
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[[Category: kreb's cycle]] | [[Category: kreb's cycle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:22:09 2008'' |
Revision as of 13:22, 20 March 2008
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| , resolution 2.19Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Fumarate hydratase, with EC number 4.2.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of apo fumarase C from Escherichia coli
Overview
Previous crystal structures of fumarase C from Escherichia coli have noted two occupied dicarboxylate-binding sites termed the active site and the B site. Here, the first known fumarase C structure is reported in which both sites are unoccupied by bound ligand. This so-called ;free' crystal form shows conservation of the active-site water in a similar orientation to that reported in other fumarase C crystal structures. More importantly, a shift of His129 has been observed at the B site. This new crystallographic information suggests the use of water as a permanent member of the active site and the use of an imidazole-imidazolium conversion to control access at the allosteric B site.
About this Structure
1YFE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of free fumarase C from Escherichia coli., Weaver T, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1395-401. Epub, 2005 Sep 28. PMID:16204892
Page seeded by OCA on Thu Mar 20 15:22:09 2008
