1ygm
From Proteopedia
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| - | [[Image:1ygm.gif|left|200px]] | + | [[Image:1ygm.gif|left|200px]] |
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| - | '''NMR structure of Mistic''' | + | {{Structure |
| + | |PDB= 1ygm |SIZE=350|CAPTION= <scene name='initialview01'>1ygm</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 Bacillus subtilis subsp. subtilis str. 168]) | ||
| + | }} | ||
| + | |||
| + | '''NMR structure of Mistic''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YGM is a [ | + | 1YGM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. |
==Reference== | ==Reference== | ||
| - | NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:[http:// | + | NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15731457 15731457] |
[[Category: Bacillus subtilis subsp. subtilis str. 168]] | [[Category: Bacillus subtilis subsp. subtilis str. 168]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: integral membrane protein]] | [[Category: integral membrane protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:22:38 2008'' |
Revision as of 13:22, 20 March 2008
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| Gene: | YugO-a (Bacillus subtilis subsp. subtilis str. 168) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structure of Mistic
Overview
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
About this Structure
1YGM is a Single protein structure of sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA.
Reference
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457
Page seeded by OCA on Thu Mar 20 15:22:38 2008
