1yhn
From Proteopedia
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| - | [[Image:1yhn.gif|left|200px]] | + | [[Image:1yhn.gif|left|200px]] |
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| - | '''Structure basis of RILP recruitment by Rab7''' | + | {{Structure |
| + | |PDB= 1yhn |SIZE=350|CAPTION= <scene name='initialview01'>1yhn</scene>, resolution 3.Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= Rab7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RILP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Structure basis of RILP recruitment by Rab7''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YHN is a [ | + | 1YHN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YHN OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for recruitment of RILP by small GTPase Rab7., Wu M, Wang T, Loh E, Hong W, Song H, EMBO J. 2005 Apr 20;24(8):1491-501. Epub 2005 Mar 31. PMID:[http:// | + | Structural basis for recruitment of RILP by small GTPase Rab7., Wu M, Wang T, Loh E, Hong W, Song H, EMBO J. 2005 Apr 20;24(8):1491-501. Epub 2005 Mar 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15933719 15933719] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: rilp]] | [[Category: rilp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:22:57 2008'' |
Revision as of 13:23, 20 March 2008
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| , resolution 3.Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | Rab7 (Homo sapiens), RILP (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure basis of RILP recruitment by Rab7
Contents |
Overview
Rab7 regulates vesicle traffic from early to late endosomes, and from late endosomes to lysosomes. The crystal structure of Rab7-GTP in complex with the Rab7 binding domain of RILP reveals that Rab7 interacts with RILP specifically via two distinct areas, with the first one involving the switch and interswitch regions and the second one consisting of RabSF1 and RabSF4. Disruption of these interactions by mutations abrogates late endosomal/lysosomal targeting of Rab7 and RILP. The Rab7 binding domain of RILP forms a coiled-coil homodimer with two symmetric surfaces to interact with two separate Rab7-GTP molecules, forming a dyad configuration of Rab7-RILP(2)-Rab7. Mutations that disrupt RILP dimerization also abolish its interactions with Rab7-GTP and late endosomal/lysosomal targeting, suggesting that the dimeric form of RILP is a functional unit. Structural comparison suggests that the combined use of RabSF1 and RabSF4 with the switch regions may be a general mode of action for most Rab proteins in regulating membrane trafficking.
Disease
Known disease associated with this structure: Charcot-Marie-Tooth disease, type 2B OMIM:[602298]
About this Structure
1YHN is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for recruitment of RILP by small GTPase Rab7., Wu M, Wang T, Loh E, Hong W, Song H, EMBO J. 2005 Apr 20;24(8):1491-501. Epub 2005 Mar 31. PMID:15933719
Page seeded by OCA on Thu Mar 20 15:22:57 2008
Categories: Homo sapiens | Protein complex | Hong, W. | Song, H. | Wang, T. | Wu, M. | GTP | MG | Protein transport | Rab7 | Rilp
