This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2cfo
From Proteopedia
(New page: 200px<br /> <applet load="2cfo" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cfo, resolution 2.45Å" /> '''NON-DISCRIMINATING ...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2CFO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]] with GLU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFO OCA]]. | + | 2CFO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]] with GLU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Ligase Ligase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFO OCA]]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: protein biosynthesis]] | [[Category: protein biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:35:40 2007'' |
Revision as of 06:30, 30 October 2007
|
NON-DISCRIMINATING GLUTAMYL-TRNA SYNTHETASE FROM THERMOSYNECHOCOCCUS ELONGATUS IN COMPLEX WITH GLU
Overview
Error-free protein biosynthesis is dependent on the reliable charging of, each tRNA with its cognate amino acid. Many bacteria, however, lack a, glutaminyl-tRNA synthetase. In these organisms, tRNA(Gln) is initially, mischarged with glutamate by a non-discriminating glutamyl-tRNA synthetase, (ND-GluRS). This enzyme thus charges both tRNA(Glu) and tRNA(Gln) with, glutamate. Discriminating GluRS (D-GluRS), found in some bacteria and all, eukaryotes, exclusively generates Glu-tRNA(Glu). Here we present the first, crystal structure of a non-discriminating GluRS from Thermosynechococcus, elongatus (ND-GluRS(Tel)) in complex with glutamate at a resolution of, 2.45 A. Structurally, the enzyme shares the overall architecture of the, discriminating GluRS from Thermus thermophilus (D-GluRS(Tth)). ... [(full description)]
About this Structure
2CFO is a [Single protein] structure of sequence from [Synechococcus elongatus] with GLU as [ligand]. Active as [Ligase], with EC number [6.1.1.17]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a non-discriminating glutamyl-tRNA synthetase., Schulze JO, Masoumi A, Nickel D, Jahn M, Jahn D, Schubert WD, Heinz DW, J Mol Biol. 2006 Sep 1;361(5):888-97. Epub 2006 Jul 5. PMID:16876193
Page seeded by OCA on Tue Oct 30 08:35:40 2007
