3ejw

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[[Image:3ejw.png|left|200px]]
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==Crystal Structure of the Sinorhizobium meliloti AI-2 receptor, SmLsrB==
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<StructureSection load='3ejw' size='340' side='right' caption='[[3ejw]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ejw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EJW FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAV:(2R,4S)-2-METHYL-2,3,3,4-TETRAHYDROXYTETRAHYDROFURAN'>PAV</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tjy|1tjy]], [[1jx6|1jx6]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AitB, RB0590, SM_b21016, SMb21016 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=382 Sinorhizobium meliloti])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ejw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ejw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ejw RCSB], [http://www.ebi.ac.uk/pdbsum/3ejw PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/3ejw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many bacterial species respond to the quorum-sensing signal autoinducer-2 (AI-2) by regulating different niche-specific genes. Here, we show that Sinorhizobium meliloti, a plant symbiont lacking the gene for the AI-2 synthase, while not capable of producing AI-2 can nonetheless respond to AI-2 produced by other species. We demonstrate that S. meliloti has a periplasmic binding protein that binds AI-2. The crystal structure of this protein (here named SmlsrB) with its ligand reveals that it binds (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF), the identical AI-2 isomer recognized by LsrB of Salmonella typhimurium. The gene encoding SmlsrB is in an operon with orthologues of the lsr genes required for AI-2 internalization in enteric bacteria. Accordingly, S. meliloti internalizes exogenous AI-2, and mutants in this operon are defective in AI-2 internalization. S. meliloti does not gain a metabolic benefit from internalizing AI-2, suggesting that AI-2 functions as a signal in S. meliloti. Furthermore, S. meliloti can completely eliminate the AI-2 secreted by Erwinia carotovora, a plant pathogen shown to use AI-2 to regulate virulence. Our findings suggest that S. meliloti is capable of 'eavesdropping' on the AI-2 signalling of other species and interfering with AI-2-regulated behaviours such as virulence.
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{{STRUCTURE_3ejw| PDB=3ejw | SCENE= }}
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Sinorhizobium meliloti, a bacterium lacking the autoinducer-2 (AI-2) synthase, responds to AI-2 supplied by other bacteria.,Pereira CS, McAuley JR, Taga ME, Xavier KB, Miller ST Mol Microbiol. 2008 Dec;70(5):1223-35. PMID:18990189<ref>PMID:18990189</ref>
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===Crystal Structure of the Sinorhizobium meliloti AI-2 receptor, SmLsrB===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18990189}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3ejw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EJW OCA].
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</StructureSection>
[[Category: Sinorhizobium meliloti]]
[[Category: Sinorhizobium meliloti]]
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[[Category: McAuley, J R.]]
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[[Category: McAuley, J R]]
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[[Category: Miller, S T.]]
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[[Category: Miller, S T]]
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[[Category: Pereira, C.]]
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[[Category: Pereira, C]]
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[[Category: Taga, M E.]]
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[[Category: Taga, M E]]
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[[Category: Xavier, K B.]]
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[[Category: Xavier, K B]]
[[Category: Periplasmic binding protein]]
[[Category: Periplasmic binding protein]]
[[Category: Signaling protein]]
[[Category: Signaling protein]]

Revision as of 13:33, 19 November 2014

Crystal Structure of the Sinorhizobium meliloti AI-2 receptor, SmLsrB

3ejw, resolution 1.80Å

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