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1yov

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[[Image:1yov.gif|left|200px]]<br /><applet load="1yov" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1yov.gif|left|200px]]
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caption="1yov, resolution 2.6&Aring;" />
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'''Insights into the Ubiquitin Transfer Cascade from the refined structure of the activating enzyme for NEDD8'''<br />
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{{Structure
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|PDB= 1yov |SIZE=350|CAPTION= <scene name='initialview01'>1yov</scene>, resolution 2.6&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY=
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|GENE= APPBP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), UBE1C, UBA3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Insights into the Ubiquitin Transfer Cascade from the refined structure of the activating enzyme for NEDD8'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1YOV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1NGV. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOV OCA].
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1YOV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry 1NGV. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOV OCA].
==Reference==
==Reference==
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Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8., Walden H, Podgorski MS, Schulman BA, Nature. 2003 Mar 20;422(6929):330-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12646924 12646924]
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Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8., Walden H, Podgorski MS, Schulman BA, Nature. 2003 Mar 20;422(6929):330-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12646924 12646924]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: ubiquitin]]
[[Category: ubiquitin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:25:37 2008''

Revision as of 13:25, 20 March 2008

Image:1yov.gif


PDB ID 1yov

Drag the structure with the mouse to rotate
, resolution 2.6Å
Ligands:
Gene: APPBP1 (Homo sapiens), UBE1C, UBA3 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Insights into the Ubiquitin Transfer Cascade from the refined structure of the activating enzyme for NEDD8


Overview

Post-translational modification by ubiquitin-like proteins (Ublps) is an essential cellular regulatory mechanism. The Ublp NEDD8 regulates cell division, signalling and embryogenesis. Ublps are conjugated to their targets by the sequential action of E1, E2 and often E3 enzymes. Each Ublp has a dedicated E1, or activating enzyme, that initiates its conjugation cascade. First, E1 associates with the Ublp and catalyses adenylation of the carboxy terminus of the Ublp. Second, E1 forms a thioester between its catalytic cysteine and the Ublp. Next, E1 is loaded with a second Ublp molecule, adenylating the C terminus of this second Ublp while still carrying the first thioester-bound Ublp. Last, E1 binds E2 and promotes Ublp transfer to the catalytic cysteine of E2. We report here the structure and mutational analysis of human APPBP1-UBA3, the heterodimeric E1 enzyme for NEDD8 (ref. 11). Each E1 activity is specified by a domain: an adenylation domain resembling bacterial adenylating enzymes, an E1-specific domain organized around the catalytic cysteine, and a domain involved in E2 recognition resembling ubiquitin. The domains are arranged around two clefts that coordinate protein and nucleotide binding so that each of E1's reactions drives the next, in an assembly-line fashion.

About this Structure

1YOV is a Protein complex structure of sequences from Homo sapiens. This structure supersedes the now removed PDB entry 1NGV. Full crystallographic information is available from OCA.

Reference

Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8., Walden H, Podgorski MS, Schulman BA, Nature. 2003 Mar 20;422(6929):330-4. PMID:12646924

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