1yqs
From Proteopedia
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| - | [[Image:1yqs.gif|left|200px]] | + | [[Image:1yqs.gif|left|200px]] |
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| - | '''Inhibition of the R61 DD-Peptidase by N-benzoyl-beta-sultam''' | + | {{Structure |
| + | |PDB= 1yqs |SIZE=350|CAPTION= <scene name='initialview01'>1yqs</scene>, resolution 1.05Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BSA:2-(BENZOYLAMINO)ETHANESULFONIC+ACID'>BSA</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Serine-type_D-Ala-D-Ala_carboxypeptidase Serine-type D-Ala-D-Ala carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.16.4 3.4.16.4] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Inhibition of the R61 DD-Peptidase by N-benzoyl-beta-sultam''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YQS is a [ | + | 1YQS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YQS OCA]. |
==Reference== | ==Reference== | ||
| - | Inactivation of bacterial DD-peptidase by beta-sultams., Llinas A, Ahmed N, Cordaro M, Laws AP, Frere JM, Delmarcelle M, Silvaggi NR, Kelly JA, Page MI, Biochemistry. 2005 May 31;44(21):7738-46. PMID:[http:// | + | Inactivation of bacterial DD-peptidase by beta-sultams., Llinas A, Ahmed N, Cordaro M, Laws AP, Frere JM, Delmarcelle M, Silvaggi NR, Kelly JA, Page MI, Biochemistry. 2005 May 31;44(21):7738-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15909988 15909988] |
[[Category: Serine-type D-Ala-D-Ala carboxypeptidase]] | [[Category: Serine-type D-Ala-D-Ala carboxypeptidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sulfonylation]] | [[Category: sulfonylation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:26:18 2008'' |
Revision as of 13:26, 20 March 2008
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| , resolution 1.05Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Serine-type D-Ala-D-Ala carboxypeptidase, with EC number 3.4.16.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Inhibition of the R61 DD-Peptidase by N-benzoyl-beta-sultam
Overview
N-Acyl-beta-sultams are time-dependent, irreversible active site-directed inhibitors of Streptomyces R61 DD-peptidase. The rate of inactivation is first order with respect to beta-sultam concentration, and the second-order rate constants show a dependence on pH similar to that for the hydrolysis of a substrate. Inactivation is due to the formation of a stable 1:1 enzyme-inhibitor complex as a result of the active site serine being sulfonylated by the beta-sultam as shown by ESI-MS analysis and by X-ray crystallography. A striking feature of the sulfonyl enzyme is that the inhibitor is not bound to the oxyanion hole but interacts extensively with the "roof" of the active site where the Arg 285 is located.
About this Structure
1YQS is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.
Reference
Inactivation of bacterial DD-peptidase by beta-sultams., Llinas A, Ahmed N, Cordaro M, Laws AP, Frere JM, Delmarcelle M, Silvaggi NR, Kelly JA, Page MI, Biochemistry. 2005 May 31;44(21):7738-46. PMID:15909988
Page seeded by OCA on Thu Mar 20 15:26:18 2008
