4g51
From Proteopedia
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| - | [[ | + | ==Crystallographic analysis of the interaction of nitric oxide with hemoglobin from Trematomus bernacchii in the T quaternary structure (fully ligated state).== |
| + | <StructureSection load='4g51' size='340' side='right' caption='[[4g51]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4g51]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trematomus_bernacchii Trematomus bernacchii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G51 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g51 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g51 RCSB], [http://www.ebi.ac.uk/pdbsum/4g51 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/HBA_TREBE HBA_TREBE]] Involved in oxygen transport from gills to the various peripheral tissues. [[http://www.uniprot.org/uniprot/HBB_TREBE HBB_TREBE]] Involved in oxygen transport from gills to the various peripheral tissues. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Despite their high physiological relevance, haemoglobin crystal structures with NO bound to haem constitute less than 1% of the total ligated haemoglobins (Hbs) deposited in the Protein Data Bank. The major difficulty in obtaining NO-ligated Hbs is most likely to be related to the oxidative denitrosylation caused by the high reactivity of the nitrosylated species with O(2). Here, using Raman-assisted X-ray crystallography, it is shown that under X-ray exposure (at four different radiation doses) crystals of nitrosylated haemoglobin from Trematomus bernacchii undergo a transition, mainly in the beta chains, that generates a pentacoordinate species owing to photodissociation of the Fe-NO bond. These data provide a physical explanation for the low number of nitrosylated Hb structures available in the literature. | ||
| - | + | Selective X-ray-induced NO photodissociation in haemoglobin crystals: evidence from a Raman-assisted crystallographic study.,Merlino A, Fuchs MR, Pica A, Balsamo A, Dworkowski FS, Pompidor G, Mazzarella L, Vergara A Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):137-40. doi:, 10.1107/S0907444912042229. Epub 2012 Dec 20. PMID:23275172<ref>PMID:23275172</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[Hemoglobin 3D structures|Hemoglobin 3D structures]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Trematomus bernacchii]] | [[Category: Trematomus bernacchii]] | ||
| - | [[Category: Balsamo, A | + | [[Category: Balsamo, A]] |
| - | [[Category: Mazzarella, L | + | [[Category: Mazzarella, L]] |
| - | [[Category: Merlino, A | + | [[Category: Merlino, A]] |
| - | [[Category: Pica, A | + | [[Category: Pica, A]] |
| - | [[Category: Vergara, A | + | [[Category: Vergara, A]] |
[[Category: All alpha protein]] | [[Category: All alpha protein]] | ||
[[Category: N-terminal acetylated serine]] | [[Category: N-terminal acetylated serine]] | ||
[[Category: Oxygen transport]] | [[Category: Oxygen transport]] | ||
[[Category: Oxygen transporter]] | [[Category: Oxygen transporter]] | ||
Revision as of 13:26, 25 December 2014
Crystallographic analysis of the interaction of nitric oxide with hemoglobin from Trematomus bernacchii in the T quaternary structure (fully ligated state).
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