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1yso

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[[Image:1yso.jpg|left|200px]]<br /><applet load="1yso" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1yso.jpg|left|200px]]
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caption="1yso, resolution 1.73&Aring;" />
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'''YEAST CU, ZN SUPEROXIDE DISMUTASE WITH THE REDUCED BRIDGE BROKEN'''<br />
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{{Structure
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|PDB= 1yso |SIZE=350|CAPTION= <scene name='initialview01'>1yso</scene>, resolution 1.73&Aring;
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|SITE= <scene name='pdbsite=CU:Cu+Coordination+Site+Also+The+Enzyme+Catalytic+Site'>CU</scene> and <scene name='pdbsite=ZN:Zn+Coordination+Site'>ZN</scene>
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|LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1]
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|GENE=
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}}
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'''YEAST CU, ZN SUPEROXIDE DISMUTASE WITH THE REDUCED BRIDGE BROKEN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1YSO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with <scene name='pdbligand=CU1:'>CU1</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Known structural/functional Sites: <scene name='pdbsite=CU:Cu+Coordination+Site+Also+The+Enzyme+Catalytic+Site'>CU</scene> and <scene name='pdbsite=ZN:Zn+Coordination+Site'>ZN</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YSO OCA].
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1YSO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YSO OCA].
==Reference==
==Reference==
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Unusual trigonal-planar copper configuration revealed in the atomic structure of yeast copper-zinc superoxide dismutase., Ogihara NL, Parge HE, Hart PJ, Weiss MS, Goto JJ, Crane BR, Tsang J, Slater K, Roe JA, Valentine JS, Eisenberg D, Tainer JA, Biochemistry. 1996 Feb 20;35(7):2316-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8652572 8652572]
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Unusual trigonal-planar copper configuration revealed in the atomic structure of yeast copper-zinc superoxide dismutase., Ogihara NL, Parge HE, Hart PJ, Weiss MS, Goto JJ, Crane BR, Tsang J, Slater K, Roe JA, Valentine JS, Eisenberg D, Tainer JA, Biochemistry. 1996 Feb 20;35(7):2316-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8652572 8652572]
[[Category: Candida albicans]]
[[Category: Candida albicans]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: zinc]]
[[Category: zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:08:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:27:03 2008''

Revision as of 13:27, 20 March 2008

Image:1yso.jpg


PDB ID 1yso

Drag the structure with the mouse to rotate
, resolution 1.73Å
Sites: and
Ligands: and
Activity: Superoxide dismutase, with EC number 1.15.1.1
Coordinates: save as pdb, mmCIF, xml



YEAST CU, ZN SUPEROXIDE DISMUTASE WITH THE REDUCED BRIDGE BROKEN


Overview

The three-dimensional structure of yeast copper-zinc superoxide dismutase (CuZnSOD) has been determined in a new crystal form in space group R32 and refined against X-ray diffraction data using difference Fourier and restrained crystallographic refinement techniques. The unexpected result is that the copper ion has moved approximately 1 angstrom from its position in previously reported CuZnSOD models, the copper-imidazolate bridge is broken, and a roughly trigonal planar ligand geometry characteristic of Cu(I) rather than Cu(II) is revealed. Final R values for the two nearly identical room temperature structures are 18.6% for all 19 149 reflections in the 10.0-1.7 angstrom resolution range and 18. 2% for 17 682 reflections (F > 2 sigma) in the 10.0-1.73 angstrom resolution range. A third structure has been determined using X-ray data collected at -180 degrees C. The final R value for this structure is 19.0% (R(free) = 22.9%) for all 24 356 reflections in the 10.0-1.55 angstrom resolution range. Virtually no change in the positions of the ligands to the zinc center is observed in these models. The origin of the broken bridge and altered Cu-ligand geometry is discussed.

About this Structure

1YSO is a Single protein structure of sequence from Candida albicans. Full crystallographic information is available from OCA.

Reference

Unusual trigonal-planar copper configuration revealed in the atomic structure of yeast copper-zinc superoxide dismutase., Ogihara NL, Parge HE, Hart PJ, Weiss MS, Goto JJ, Crane BR, Tsang J, Slater K, Roe JA, Valentine JS, Eisenberg D, Tainer JA, Biochemistry. 1996 Feb 20;35(7):2316-21. PMID:8652572

Page seeded by OCA on Thu Mar 20 15:27:03 2008

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