1yvp
From Proteopedia
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| - | [[Image:1yvp.gif|left|200px]] | + | [[Image:1yvp.gif|left|200px]] |
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| - | '''Ro autoantigen complexed with RNAs''' | + | {{Structure |
| + | |PDB= 1yvp |SIZE=350|CAPTION= <scene name='initialview01'>1yvp</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Ro autoantigen complexed with RNAs''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YVP is a [ | + | 1YVP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YVP OCA]. |
==Reference== | ==Reference== | ||
| - | Structural insights into RNA quality control: the Ro autoantigen binds misfolded RNAs via its central cavity., Stein AJ, Fuchs G, Fu C, Wolin SL, Reinisch KM, Cell. 2005 May 20;121(4):529-39. PMID:[http:// | + | Structural insights into RNA quality control: the Ro autoantigen binds misfolded RNAs via its central cavity., Stein AJ, Fuchs G, Fu C, Wolin SL, Reinisch KM, Cell. 2005 May 20;121(4):529-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15907467 15907467] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
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[[Category: heat repeat; von willebrand factor a; rossmann fold; midas motif; rna duplex; ssrna]] | [[Category: heat repeat; von willebrand factor a; rossmann fold; midas motif; rna duplex; ssrna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:28:05 2008'' |
Revision as of 13:28, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Ro autoantigen complexed with RNAs
Overview
The Ro 60 kDa autoantigen is a major target of the immune response in patients with systemic lupus erythematosus. In vertebrate cells, Ro binds misfolded small RNAs and likely functions in RNA quality control. In eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs. We present structures of unliganded Ro and Ro complexed with two RNAs at 1.95 and 2.2 A resolution, respectively. Ro consists of a von Willebrand factor A domain and a doughnut-shaped domain composed of HEAT repeats. In the complex, a fragment of Y RNA binds on the outer surface of the HEAT-repeat ring, and single-stranded RNA binds in the toroid hole. Mutagenesis supports a binding site for misfolded RNAs that encompasses both sites, with a single-stranded end inserted into the toroid cavity. Our experiments suggest that one role of Y RNAs may be to regulate access of other RNAs to Ro.
About this Structure
1YVP is a Single protein structure of sequence from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Structural insights into RNA quality control: the Ro autoantigen binds misfolded RNAs via its central cavity., Stein AJ, Fuchs G, Fu C, Wolin SL, Reinisch KM, Cell. 2005 May 20;121(4):529-39. PMID:15907467
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