1yyn
From Proteopedia
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| - | [[Image:1yyn.gif|left|200px]] | + | [[Image:1yyn.gif|left|200px]] |
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| - | '''A common binding site for disialyllactose and a tri-peptide in the C-fragment of tetanus neurotoxin''' | + | {{Structure |
| + | |PDB= 1yyn |SIZE=350|CAPTION= <scene name='initialview01'>1yyn</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''A common binding site for disialyllactose and a tri-peptide in the C-fragment of tetanus neurotoxin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YYN is a [ | + | 1YYN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYN OCA]. |
==Reference== | ==Reference== | ||
| - | Common binding site for disialyllactose and tri-peptide in C-fragment of tetanus neurotoxin., Jayaraman S, Eswaramoorthy S, Kumaran D, Swaminathan S, Proteins. 2005 Nov 1;61(2):288-95. PMID:[http:// | + | Common binding site for disialyllactose and tri-peptide in C-fragment of tetanus neurotoxin., Jayaraman S, Eswaramoorthy S, Kumaran D, Swaminathan S, Proteins. 2005 Nov 1;61(2):288-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16104015 16104015] |
[[Category: Clostridium tetani]] | [[Category: Clostridium tetani]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ganglioside]] | [[Category: ganglioside]] | ||
[[Category: gd3]] | [[Category: gd3]] | ||
| - | [[Category: | + | [[Category: inhibitor]] |
[[Category: tetanus toxin]] | [[Category: tetanus toxin]] | ||
[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:29:07 2008'' |
Revision as of 13:29, 20 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Tentoxilysin, with EC number 3.4.24.68 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A common binding site for disialyllactose and a tri-peptide in the C-fragment of tetanus neurotoxin
Overview
Clostridial neurotoxins are comprised of botulinum (BoNT) and tetanus (TeNT), which share significant structural and functional similarity. Crystal structures of the binding domain of TeNT complexed with disialyllactose (DiSia) and a tri-peptide Tyr-Glu-Trp (YEW) have been determined to 2.3 and 2.2 A, respectively. Both DiSia and YEW bind in a shallow cleft region on the surface of the molecule in the beta-trefoil domain, interacting with a set of common residues, Asp1147, Asp1214, Asn1216, and Arg1226. DiSia and YEW binding at the same site in tetanus toxin provides a putative site that could be occupied either by a ganglioside moiety or a peptide. Soaking experiments with a mixture of YEW and DiSia show that YEW competes with DiSia, suggesting that YEW can be used to block ganglioside binding. A comparison with the TeNT binding domain in complex with small molecules, BoNT/A and /B, provides insight into the different modes of ganglioside binding.
About this Structure
1YYN is a Single protein structure of sequence from Clostridium tetani. Full crystallographic information is available from OCA.
Reference
Common binding site for disialyllactose and tri-peptide in C-fragment of tetanus neurotoxin., Jayaraman S, Eswaramoorthy S, Kumaran D, Swaminathan S, Proteins. 2005 Nov 1;61(2):288-95. PMID:16104015
Page seeded by OCA on Thu Mar 20 15:29:07 2008
