1z00
From Proteopedia
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| - | [[Image:1z00.gif|left|200px]] | + | [[Image:1z00.gif|left|200px]] |
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| - | '''Solution structure of the C-terminal domain of ERCC1 complexed with the C-terminal domain of XPF''' | + | {{Structure |
| + | |PDB= 1z00 |SIZE=350|CAPTION= <scene name='initialview01'>1z00</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Solution structure of the C-terminal domain of ERCC1 complexed with the C-terminal domain of XPF''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z00 is a [ | + | 1Z00 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z00 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of the human ERCC1/XPF interaction domains reveals a complementary role for the two proteins in nucleotide excision repair., Tripsianes K, Folkers G, Ab E, Das D, Odijk H, Jaspers NG, Hoeijmakers JH, Kaptein R, Boelens R, Structure. 2005 Dec;13(12):1849-58. PMID:[http:// | + | The structure of the human ERCC1/XPF interaction domains reveals a complementary role for the two proteins in nucleotide excision repair., Tripsianes K, Folkers G, Ab E, Das D, Odijk H, Jaspers NG, Hoeijmakers JH, Kaptein R, Boelens R, Structure. 2005 Dec;13(12):1849-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16338413 16338413] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: helix-hairpin-helix]] | [[Category: helix-hairpin-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:29:42 2008'' |
Revision as of 13:29, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the C-terminal domain of ERCC1 complexed with the C-terminal domain of XPF
Contents |
Overview
The human ERCC1/XPF complex is a structure-specific endonuclease with defined polarity that participates in multiple DNA repair pathways. We report the heterodimeric structure of the C-terminal domains of both proteins responsible for ERCC1/XPF complex formation. Both domains exhibit the double helix-hairpin-helix motif (HhH)2, and they are related by a pseudo-2-fold symmetry axis. In the XPF domain, the hairpin of the second motif is replaced by a short turn. The ERCC1 domain folds properly only in the presence of the XPF domain, which implies a role for XPF as a scaffold for the folding of ERCC1. The intersubunit interactions are largely hydrophobic in nature. NMR titration data show that only the ERCC1 domain of the ERCC1/XPF complex is involved in DNA binding. On the basis of these findings, we propose a model for the targeting of XPF nuclease via ERCC1-mediated interactions in the context of nucleotide excision repair.
Disease
Known diseases associated with this structure: Cerebrooculofacioskeletal syndrome 4 OMIM:[126380], XFE progeroid syndrome OMIM:[133520], Xeroderma pigmentosum, group F OMIM:[133520]
About this Structure
1Z00 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The structure of the human ERCC1/XPF interaction domains reveals a complementary role for the two proteins in nucleotide excision repair., Tripsianes K, Folkers G, Ab E, Das D, Odijk H, Jaspers NG, Hoeijmakers JH, Kaptein R, Boelens R, Structure. 2005 Dec;13(12):1849-58. PMID:16338413
Page seeded by OCA on Thu Mar 20 15:29:42 2008
