4hhh
From Proteopedia
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| - | [[ | + | ==Structure of Pisum sativum Rubisco== |
| + | <StructureSection load='4hhh' size='340' side='right' caption='[[4hhh]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4hhh]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HHH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HHH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RUB:RIBULOSE-1,5-DIPHOSPHATE'>RUB</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hhh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hhh RCSB], [http://www.ebi.ac.uk/pdbsum/4hhh PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The first structure of a ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from a pulse crop is reported. Rubisco was purified from Pisum sativum (garden pea) and diffraction-quality crystals were obtained by hanging-drop vapour diffusion in the presence of the substrate ribulose 1,5-bisphosphate. X-ray diffraction data were recorded to 2.20 A resolution from a single crystal at the Canadian Light Source. The overall quaternary structure of non-activated P. sativum Rubisco highlights the conservation of the form I Rubisco hexadecameric complex. The electron density places the substrate in the active site at the interface of the large-subunit dimers. Lys201 in the active site is not carbamylated as expected for this non-activated structure. Some heterogeneity in the small-subunit sequence is noted, as well as possible variations in the conformation and contacts of ribulose 1,5-bisphosphate in the large-subunit active sites. Overall, the active-site conformation most closely correlates with the `closed' conformation observed in other substrate/inhibitor-bound Rubisco structures. | ||
| - | + | Structure of Pisum sativum Rubisco with bound ribulose 1,5-bisphosphate.,Loewen PC, Didychuk AL, Switala J, Perez-Luque R, Fita I, Loewen MC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jan 1;69(Pt 1):10-4. doi: , 10.1107/S1744309112047549. Epub 2012 Dec 25. PMID:23295478<ref>PMID:23295478</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[RuBisCO|RuBisCO]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Pisum sativum]] | [[Category: Pisum sativum]] | ||
[[Category: Ribulose-bisphosphate carboxylase]] | [[Category: Ribulose-bisphosphate carboxylase]] | ||
| - | [[Category: Didychuk, A L | + | [[Category: Didychuk, A L]] |
| - | [[Category: Loewen, M C | + | [[Category: Loewen, M C]] |
| - | [[Category: Loewen, P C | + | [[Category: Loewen, P C]] |
| - | [[Category: Switala, J | + | [[Category: Switala, J]] |
[[Category: 5-bisphosphate]] | [[Category: 5-bisphosphate]] | ||
[[Category: Lyase]] | [[Category: Lyase]] | ||
[[Category: Ribulose-1]] | [[Category: Ribulose-1]] | ||
[[Category: Rubisco]] | [[Category: Rubisco]] | ||
Revision as of 09:19, 10 December 2014
Structure of Pisum sativum Rubisco
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