2lcl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2lcl.png|left|200px]]
+
==Solution Structure of RfaH carboxyterminal domain==
 +
<StructureSection load='2lcl' size='340' side='right' caption='[[2lcl]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2lcl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LCL FirstGlance]. <br>
 +
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rfaH, hlyT, sfrB, Z5362, ECs4770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lcl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lcl RCSB], [http://www.ebi.ac.uk/pdbsum/2lcl PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
NusG homologs regulate transcription and coupled processes in all living organisms. The Escherichia coli (E. coli) two-domain paralogs NusG and RfaH have conformationally identical N-terminal domains (NTDs) but dramatically different carboxy-terminal domains (CTDs), a beta barrel in NusG and an alpha hairpin in RfaH. Both NTDs interact with elongating RNA polymerase (RNAP) to reduce pausing. In NusG, NTD and CTD are completely independent, and NusG-CTD interacts with termination factor Rho or ribosomal protein S10. In contrast, RfaH-CTD makes extensive contacts with RfaH-NTD to mask an RNAP-binding site therein. Upon RfaH interaction with its DNA target, the operon polarity suppressor (ops) DNA, RfaH-CTD is released, allowing RfaH-NTD to bind to RNAP. Here, we show that the released RfaH-CTD completely refolds from an all-alpha to an all-beta conformation identical to that of NusG-CTD. As a consequence, RfaH-CTD binding to S10 is enabled and translation of RfaH-controlled operons is strongly potentiated. PAPERFLICK:
-
{{STRUCTURE_2lcl| PDB=2lcl | SCENE= }}
+
An alpha helix to beta barrel domain switch transforms the transcription factor RfaH into a translation factor.,Burmann BM, Knauer SH, Sevostyanova A, Schweimer K, Mooney RA, Landick R, Artsimovitch I, Rosch P Cell. 2012 Jul 20;150(2):291-303. doi: 10.1016/j.cell.2012.05.042. PMID:22817892<ref>PMID:22817892</ref>
-
===Solution Structure of RfaH carboxyterminal domain===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
-
{{ABSTRACT_PUBMED_22817892}}
+
==See Also==
-
 
+
*[[Transcriptional activator|Transcriptional activator]]
-
==About this Structure==
+
== References ==
-
[[2lcl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LCL OCA].
+
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli o157:h7]]
[[Category: Escherichia coli o157:h7]]
[[Category: Burmann, B M.]]
[[Category: Burmann, B M.]]

Revision as of 09:27, 5 November 2014

Solution Structure of RfaH carboxyterminal domain

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lcl"
Personal tools