1z7h
From Proteopedia
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| - | [[Image:1z7h.gif|left|200px]] | + | [[Image:1z7h.gif|left|200px]] |
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| - | '''2.3 Angstrom crystal structure of tetanus neurotoxin light chain''' | + | {{Structure |
| + | |PDB= 1z7h |SIZE=350|CAPTION= <scene name='initialview01'>1z7h</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] | ||
| + | |GENE= tetX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1513 Clostridium tetani]) | ||
| + | }} | ||
| + | |||
| + | '''2.3 Angstrom crystal structure of tetanus neurotoxin light chain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z7H is a [ | + | 1Z7H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7H OCA]. |
==Reference== | ==Reference== | ||
| - | 2.3 A crystal structure of tetanus neurotoxin light chain., Breidenbach MA, Brunger AT, Biochemistry. 2005 May 24;44(20):7450-7. PMID:[http:// | + | 2.3 A crystal structure of tetanus neurotoxin light chain., Breidenbach MA, Brunger AT, Biochemistry. 2005 May 24;44(20):7450-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15895988 15895988] |
[[Category: Clostridium tetani]] | [[Category: Clostridium tetani]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tetanus]] | [[Category: tetanus]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:32:07 2008'' |
Revision as of 13:32, 20 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | tetX (Clostridium tetani) | ||||||
| Activity: | Tentoxilysin, with EC number 3.4.24.68 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
2.3 Angstrom crystal structure of tetanus neurotoxin light chain
Overview
TeNT is the causative agent of the neuroparalytic disease tetanus. A key component of TeNT is its light chain, a Zn(2+) endopeptidase that targets SNAREs. Recent structural studies of closely related BoNT endopeptidases indicate that substrate-binding exosites remote from a conserved active site are the primary determinants of substrate specificity. Here we report the 2.3 A X-ray crystal structure of TeNT-LC, determined by combined molecular replacement and MAD phasing. As expected, the overall structure of TeNT-LC is similar to the other known CNT light chain structures, including a conserved thermolysin-like core inserted between structurally distinct amino- and carboxy-terminal regions. Differences between TeNT-LC and the other CNT light chains are mainly limited to surface features such as unique electrostatic potential profiles. An analysis of surface residue conservation reveals a pattern of relatively high variability matching the path of substrate binding around BoNT/A, possibly serving to accommodate the variations in different SNARE targets of the CNT group.
About this Structure
1Z7H is a Single protein structure of sequence from Clostridium tetani. Full crystallographic information is available from OCA.
Reference
2.3 A crystal structure of tetanus neurotoxin light chain., Breidenbach MA, Brunger AT, Biochemistry. 2005 May 24;44(20):7450-7. PMID:15895988
Page seeded by OCA on Thu Mar 20 15:32:07 2008
