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DNA ligase

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'''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated , in a species-dependent manner, by hydrolysis of ATP or NAD+. Mammalian LigD I ligates the nascent DNA of the lagging strand; LigD III complexes with XRCC1 in the process of nucleotide excision repair; LigD IV complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining.
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'''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated , in a species-dependent manner, by hydrolysis of ATP or NAD+. Mammalian LigD I ligates the nascent DNA of the lagging strand; LigD III complexes with XRCC1 in the process of nucleotide excision repair; LigD IV complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining.LigD contains several domains: adenylation domain; DNA-binding domain and the ca. 100 amino acid long BRCT motif.
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== 3D Structures of DNA ligase ==
== 3D Structures of DNA ligase ==
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''Update June 2012''
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''Update January 2013''
===ATP-dependent LigD===
===ATP-dependent LigD===
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[[2cfm]] - LigD + AMP – ''Pyrococcus furiosus''<br />
[[2cfm]] - LigD + AMP – ''Pyrococcus furiosus''<br />
[[2fao]] - PaLigD polymerase domain – ''Pseudomonas aeruginosa''<br />
[[2fao]] - PaLigD polymerase domain – ''Pseudomonas aeruginosa''<br />
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[[2faq]], [[ 2far]] - PaLigD polymerase domain + ATP + Mn<br />
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[[2faq]], [[2far]] - PaLigD polymerase domain + ATP + Mn<br />
[[1a0i]] – LigD + ATP – Bacteriophage T7<br />
[[1a0i]] – LigD + ATP – Bacteriophage T7<br />
[[4eq5]] – LigD + AMP – ''Thermococcus sibiricus''
[[4eq5]] – LigD + AMP – ''Thermococcus sibiricus''
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===NAD+-dependent LigD===
===NAD+-dependent LigD===
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[[3jsl]], [[ 3jsn]] - LigD adenylation domain – ''Staphylococcus aureus''<br />
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[[3jsl]], [[3jsn]] - LigD adenylation domain – ''Staphylococcus aureus''<br />
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[[3ba8]], [[ 3ba9]], [[ 3baa]], [[ 3bab]], [[ 3bac]] - EfLigD adenylation domain + inhibitor – ''Enterococcus faecalis''<br />
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[[3ba8]], [[3ba9]], [[3baa]], [[3bab]], [[3bac]] - EfLigD adenylation domain + inhibitor – ''Enterococcus faecalis''<br />
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[[1ta8]], [[ 1tae]] - EfLigD adenylation domain<br />
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[[1ta8]], [[1tae]] - EfLigD adenylation domain<br />
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[[1zau]] - LigD adenylation domain – ''Mycobacterium tuberculosis''<br />
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[[1zau]] - MtLigD adenylation domain – ''Mycobacterium tuberculosis''<br />
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[[1v9p]], [[ 1dgs]] – LigD – ''Thermos filiformis''<br />
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[[3sgi]] – MtLigD (mutant) + AMP<br />
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[[1b04]] – LigD adenylation domain (mutant) – ''Geobacillus stearothermophilus''
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[[1v9p]], [[1dgs]] – LigD – ''Thermos filiformis''<br />
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[[1b04]] – LigD adenylation domain (mutant) – ''Geobacillus stearothermophilus''<br />
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[[4glw]] – LigD A + NMN + inhibitor – ''Streptococcus pneumoniae''
===LigD===
===LigD===
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[[3ii6]], [[ 1ik9]] – hLigD IV C-terminal BRCT domains + DNA repair protein XRCC4<br />
[[3ii6]], [[ 1ik9]] – hLigD IV C-terminal BRCT domains + DNA repair protein XRCC4<br />
[[2e2w]] - hLigD IV BRCT domain - NMR<br />
[[2e2w]] - hLigD IV BRCT domain - NMR<br />
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[[1z56]] – LigD IV + ligase interacting factor 1 - yeast
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[[1z56]] – LigD IV + ligase interacting factor 1 - yeast<br />
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[[3vnn]] - hLigD IV adenylation domain<br />
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[[4hto]] - hLigD IV DNA-binding domain<br />
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[[4htp]] - hLigD IV DNA-binding domain + artemis protein peptide
</StructureSection>
</StructureSection>

Revision as of 12:23, 30 January 2013

ATP-dependent DNA ligase complexed with ATP 2hix

Drag the structure with the mouse to rotate

REFERENCES

Elevated expression of DNA ligase I in human cancers., Sun DY, Urrabaz R, Nguyen M, Marty J, Stringer S, Cruz E, Medina-Gundrum L, Weitman S., Clinical Cancer Research. 2001; 7(12):4143-4148.
Replication failure, genome instability, and increased cancer susceptibility in mice with a point mutation in the DNA ligase I gene., Harrison C, Ketchen AM, Redhead NJ, O'Sullivan MJ, Melton DW., Cancer Research. 2002; 62(14):4065-4074.

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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