1z8s
From Proteopedia
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| - | [[Image:1z8s.gif|left|200px]] | + | [[Image:1z8s.gif|left|200px]] |
| - | + | ||
| - | '''DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)''' | + | {{Structure |
| + | |PDB= 1z8s |SIZE=350|CAPTION= <scene name='initialview01'>1z8s</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= dnaG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]) | ||
| + | }} | ||
| + | |||
| + | '''DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z8S is a [ | + | 1Z8S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z8S OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation., Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP, Structure. 2005 Apr;13(4):609-16. PMID:[http:// | + | Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation., Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP, Structure. 2005 Apr;13(4):609-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15837199 15837199] |
[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Thirlway, J.]] | [[Category: Thirlway, J.]] | ||
[[Category: Waltho, J P.]] | [[Category: Waltho, J P.]] | ||
| - | [[Category: two alpha helical sub- | + | [[Category: two alpha helical sub-domain]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:32:33 2008'' |
Revision as of 13:32, 20 March 2008
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| Gene: | dnaG (Geobacillus stearothermophilus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)
Overview
The helicase-primase interaction is a critical event in DNA replication and is mediated by a putative helicase-interaction domain within the primase. The solution structure of the helicase-interaction domain of DnaG reveals that it is made up of two independent subdomains: an N-terminal six-helix module and a C-terminal two-helix module that contains the residues of the primase previously identified as important in the interaction with the helicase. We show that the two-helix module alone is sufficient for strong binding between the primase and the helicase but fails to activate the helicase; both subdomains are required for helicase activation. The six-helix module of the primase has only one close structural homolog, the N-terminal domain of the corresponding helicase. This surprising structural relationship, coupled with the differences in surface properties of the two molecules, suggests how the helicase-interaction domain may perturb the structure of the helicase and lead to activation.
About this Structure
1Z8S is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation., Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP, Structure. 2005 Apr;13(4):609-16. PMID:15837199
Page seeded by OCA on Thu Mar 20 15:32:33 2008
