1z9o

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[[Image:1z9o.gif|left|200px]]<br /><applet load="1z9o" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1z9o.gif|left|200px]]
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caption="1z9o, resolution 1.90&Aring;" />
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'''1.9 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain in Complex with the Rat ORP1 FFAT Motif'''<br />
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{{Structure
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|PDB= 1z9o |SIZE=350|CAPTION= <scene name='initialview01'>1z9o</scene>, resolution 1.90&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= Vapa, Vap33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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}}
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'''1.9 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain in Complex with the Rat ORP1 FFAT Motif'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1Z9O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9O OCA].
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1Z9O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9O OCA].
==Reference==
==Reference==
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Structural basis of FFAT motif-mediated ER targeting., Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT, Structure. 2005 Jul;13(7):1035-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16004875 16004875]
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Structural basis of FFAT motif-mediated ER targeting., Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT, Structure. 2005 Jul;13(7):1035-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16004875 16004875]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: vapc]]
[[Category: vapc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:32:53 2008''

Revision as of 13:32, 20 March 2008

Image:1z9o.gif


PDB ID 1z9o

Drag the structure with the mouse to rotate
, resolution 1.90Å
Gene: Vapa, Vap33 (Rattus norvegicus)
Coordinates: save as pdb, mmCIF, xml



1.9 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain in Complex with the Rat ORP1 FFAT Motif


Overview

The FFAT motif is a targeting signal responsible for localizing a number of proteins to the cytosolic surface of the endoplasmic reticulum (ER) and to the nuclear membrane. FFAT motifs bind to members of the highly conserved VAP protein family, which are tethered to the cytoplasmic face of the ER by a C-terminal transmembrane domain. We have solved crystal structures of the rat VAP-A MSP homology domain alone and in complex with an FFAT motif. The co-crystal structure was used to design a VAP mutant that disrupts rat and yeast VAP-FFAT interactions in vitro. The FFAT binding-defective mutant also blocked function of the VAP homolog Scs2p in yeast. Finally, overexpression of the FFAT binding-defective VAP in COS7 cells dramatically altered ER morphology. Our data establish the structural basis of FFAT-mediated ER targeting and suggest that FFAT-targeted proteins play an important role in determining ER morphology.

About this Structure

1Z9O is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural basis of FFAT motif-mediated ER targeting., Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT, Structure. 2005 Jul;13(7):1035-45. PMID:16004875

Page seeded by OCA on Thu Mar 20 15:32:53 2008

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