1zby
From Proteopedia
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| - | [[Image:1zby.gif|left|200px]] | + | [[Image:1zby.gif|left|200px]] |
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| - | '''High-Resolution Crystal Structure of Native (Resting) Cytochrome c Peroxidase (CcP)''' | + | {{Structure |
| + | |PDB= 1zby |SIZE=350|CAPTION= <scene name='initialview01'>1zby</scene>, resolution 1.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''High-Resolution Crystal Structure of Native (Resting) Cytochrome c Peroxidase (CcP)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZBY is a [ | + | 1ZBY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZBY OCA]. |
==Reference== | ==Reference== | ||
| - | High-resolution crystal structures and spectroscopy of native and compound I cytochrome c peroxidase., Bonagura CA, Bhaskar B, Shimizu H, Li H, Sundaramoorthy M, McRee DE, Goodin DB, Poulos TL, Biochemistry. 2003 May 20;42(19):5600-8. PMID:[http:// | + | High-resolution crystal structures and spectroscopy of native and compound I cytochrome c peroxidase., Bonagura CA, Bhaskar B, Shimizu H, Li H, Sundaramoorthy M, McRee DE, Goodin DB, Poulos TL, Biochemistry. 2003 May 20;42(19):5600-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12741816 12741816] |
[[Category: Cytochrome-c peroxidase]] | [[Category: Cytochrome-c peroxidase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: trp-cation radical]] | [[Category: trp-cation radical]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:33:38 2008'' |
Revision as of 13:33, 20 March 2008
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| , resolution 1.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Cytochrome-c peroxidase, with EC number 1.11.1.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
High-Resolution Crystal Structure of Native (Resting) Cytochrome c Peroxidase (CcP)
Overview
Cytochrome c peroxidase (CCP) is a 32.5 kDa mitochondrial intermembrane space heme peroxidase from Saccharomyces cerevisiae that reduces H(2)O(2) to 2H(2)O by oxidizing two molecules of cytochrome c (cyt c). Here we compare the 1.2 A native structure (CCP) with the 1.3 A structure of its stable oxidized reaction intermediate, Compound I (CCP1). In addition, crystals were analyzed by UV-vis absorption and electron paramagnetic resonance spectroscopies before and after data collection to determine the state of the Fe(IV) center and the cationic Trp191 radical formed in Compound I. The results show that X-ray exposure does not lead to reduction of Fe(IV) and only partial reduction of the Trp radical. A comparison of the two structures reveals subtle but important conformational changes that aid in the stabilization of the Trp191 cationic radical in Compound I. The higher-resolution data also enable a more accurate determination of changes in heme parameters. Most importantly, when one goes from resting state Fe(III) to Compound I, the His-Fe bond distance increases, the iron moves into the porphyrin plane leading to shorter pyrrole N-Fe bonds, and the Fe(IV)-O bond distance is 1.87 A, suggesting a single Fe(IV)-O bond and not the generally accepted double bond.
About this Structure
1ZBY is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
High-resolution crystal structures and spectroscopy of native and compound I cytochrome c peroxidase., Bonagura CA, Bhaskar B, Shimizu H, Li H, Sundaramoorthy M, McRee DE, Goodin DB, Poulos TL, Biochemistry. 2003 May 20;42(19):5600-8. PMID:12741816
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