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1zie
From Proteopedia
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| - | [[Image:1zie.gif|left|200px]] | + | [[Image:1zie.gif|left|200px]] |
| - | + | ||
| - | '''Hydrogenated gammaE crystallin in D2O solvent''' | + | {{Structure |
| + | |PDB= 1zie |SIZE=350|CAPTION= <scene name='initialview01'>1zie</scene>, resolution 1.44Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=DOD:DEUTERATED WATER'>DOD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Hydrogenated gammaE crystallin in D2O solvent''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZIE is a [ | + | 1ZIE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIE OCA]. |
==Reference== | ==Reference== | ||
| - | A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O., Artero JB, Hartlein M, McSweeney S, Timmins P, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1541-9. Epub 2005, Oct 19. PMID:[http:// | + | A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O., Artero JB, Hartlein M, McSweeney S, Timmins P, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1541-9. Epub 2005, Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16239733 16239733] |
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ACT]] | [[Category: ACT]] | ||
[[Category: DOD]] | [[Category: DOD]] | ||
| - | [[Category: 4 greek key | + | [[Category: 4 greek key motif]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:35:56 2008'' |
Revision as of 13:36, 20 March 2008
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| , resolution 1.44Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Hydrogenated gammaE crystallin in D2O solvent
Overview
Rat gammaE-crystallin was overexpressed, purified under different labelling conditions and crystallized and X-ray data were collected at resolutions between 1.71 and 1.36 A. The structures were determined by molecular replacement. In these structures, the cd loop of the Greek-key motif 3, which is the major structural key motif of the two phase-transition groups of gamma-crystallins, presents a double conformation. The influence of the perdeuteration on the protein structure was determined by comparison of the atomic positions and temperature factors of the different models. The perdeuterated proteins have a similar structure to their hydrogenated counterparts, but partial or full deuteration may have some effect on the atomic B-factor values.
About this Structure
1ZIE is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O., Artero JB, Hartlein M, McSweeney S, Timmins P, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1541-9. Epub 2005, Oct 19. PMID:16239733
Page seeded by OCA on Thu Mar 20 15:35:56 2008
