1zim

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[[Image:1zim.jpg|left|200px]]<br /><applet load="1zim" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1zim.jpg|left|200px]]
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caption="1zim, resolution 2.00&Aring;" />
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'''GCN4-LEUCINE ZIPPER CORE MUTANT ASN16GLN IN THE TRIMERIC STATE'''<br />
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{{Structure
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|PDB= 1zim |SIZE=350|CAPTION= <scene name='initialview01'>1zim</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''GCN4-LEUCINE ZIPPER CORE MUTANT ASN16GLN IN THE TRIMERIC STATE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1ZIM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIM OCA].
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1ZIM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIM OCA].
==Reference==
==Reference==
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Buried polar residues and structural specificity in the GCN4 leucine zipper., Gonzalez L Jr, Woolfson DN, Alber T, Nat Struct Biol. 1996 Dec;3(12):1011-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8946854 8946854]
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Buried polar residues and structural specificity in the GCN4 leucine zipper., Gonzalez L Jr, Woolfson DN, Alber T, Nat Struct Biol. 1996 Dec;3(12):1011-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8946854 8946854]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transcription regulation]]
[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:15:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:35:56 2008''

Revision as of 13:35, 20 March 2008

Image:1zim.jpg


PDB ID 1zim

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



GCN4-LEUCINE ZIPPER CORE MUTANT ASN16GLN IN THE TRIMERIC STATE


Overview

A conserved asparagine (Asn 16) buried in the interface of the GCN4 leucine zipper selectively favours the parallel, dimeric, coiled-coil structure. To test if other polar residues confer oligomerization specificity, the structural effects of Gln and Lys substitutions for Asn 16 were characterized. Like the wild-type peptide, the Asn 16Lys mutant formed exclusively dimers. In contrast, Gln 16, despite its chemical similarity to Asn, allowed the peptide to form both dimers and trimers. The Gln 16 side chain was accommodated by qualitatively different interactions in the dimer and trimer crystal structures. These findings demonstrate that the structural selectivity of polar residues results not only from the burial of polar atoms, but also depends on the complementarity of the side-chain stereochemistry with the surrounding structural environment.

About this Structure

1ZIM is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Buried polar residues and structural specificity in the GCN4 leucine zipper., Gonzalez L Jr, Woolfson DN, Alber T, Nat Struct Biol. 1996 Dec;3(12):1011-8. PMID:8946854

Page seeded by OCA on Thu Mar 20 15:35:56 2008

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