3w5k
From Proteopedia
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| - | ''' | + | ==Crystal structure of Snail1 and importin beta complex== |
| + | <StructureSection load='3w5k' size='340' side='right' caption='[[3w5k]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3w5k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W5K OCA]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KPNB1, NTF97 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNAI1, SNAH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w5k RCSB], [http://www.ebi.ac.uk/pdbsum/3w5k PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Snail contributes to the epithelial-mesenchymal transition by suppressing E-cadherin in transcription processes. The Snail C2H2-type zinc-finger (ZF) domain functions both as a nuclear localization signal which binds to importin beta directly and as a DNA-binding domain. Here, a 2.5 A resolution structure of four ZF domains of Snail1 complexed with importin beta is presented. The X-ray structure reveals that the four ZFs of Snail1 are required for tight binding to importin beta in the nuclear import of Snail1. The shape of the ZFs in the X-ray structure is reminiscent of a round snail, where ZF1 represents the head, ZF2-ZF4 the shell, showing a novel interaction mode, and the five C-terminal residues the tail. Although there are many kinds of C2H2-type ZFs which have the same fold as Snail, nuclear import by direct recognition of importin beta is observed in a limited number of C2H2-type ZF proteins such as Snail, Wt1, KLF1 and KLF8, which have the common feature of terminating in ZF domains with a short tail of amino acids. | ||
| - | + | Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin beta.,Choi S, Yamashita E, Yasuhara N, Song J, Son SY, Won YH, Hong HR, Shin YS, Sekimoto T, Park IY, Yoneda Y, Lee SJ Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):1050-60. doi:, 10.1107/S1399004714000972. Epub 2014 Mar 19. PMID:24699649<ref>PMID:24699649</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Human]] | ||
| + | [[Category: Choi, S.]] | ||
| + | [[Category: Lee, S J.]] | ||
| + | [[Category: Park, I Y.]] | ||
| + | [[Category: Sekimoto, T.]] | ||
| + | [[Category: Shin, Y S.]] | ||
| + | [[Category: Son, S Y.]] | ||
| + | [[Category: Song, J.]] | ||
| + | [[Category: Won, Y H.]] | ||
| + | [[Category: Yamashita, E.]] | ||
| + | [[Category: Yasuhara, N.]] | ||
| + | [[Category: Yoneda, Y.]] | ||
| + | [[Category: Importin beta]] | ||
| + | [[Category: Nuclear protein-metal binding protein complex]] | ||
| + | [[Category: Nuclear transport factor snail1]] | ||
| + | [[Category: Transcription repressor]] | ||
Revision as of 06:57, 7 May 2014
Crystal structure of Snail1 and importin beta complex
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