1zk6
From Proteopedia
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| - | [[Image:1zk6.gif|left|200px]] | + | [[Image:1zk6.gif|left|200px]] |
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| - | '''NMR solution structure of B. subtilis PrsA PPIase''' | + | {{Structure |
| + | |PDB= 1zk6 |SIZE=350|CAPTION= <scene name='initialview01'>1zk6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] | ||
| + | |GENE= prsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''NMR solution structure of B. subtilis PrsA PPIase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZK6 is a [ | + | 1ZK6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZK6 OCA]. |
==Reference== | ==Reference== | ||
| - | NMR solution structure and characterization of substrate binding site of the PPIase domain of PrsA protein from Bacillus subtilis., Tossavainen H, Permi P, Purhonen SL, Sarvas M, Kilpelainen I, Seppala R, FEBS Lett. 2006 Mar 20;580(7):1822-6. Epub 2006 Feb 24. PMID:[http:// | + | NMR solution structure and characterization of substrate binding site of the PPIase domain of PrsA protein from Bacillus subtilis., Tossavainen H, Permi P, Purhonen SL, Sarvas M, Kilpelainen I, Seppala R, FEBS Lett. 2006 Mar 20;580(7):1822-6. Epub 2006 Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16516208 16516208] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Peptidylprolyl isomerase]] | [[Category: Peptidylprolyl isomerase]] | ||
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[[Category: alpha/beta structure]] | [[Category: alpha/beta structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:36:29 2008'' |
Revision as of 13:36, 20 March 2008
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| Gene: | prsA (Bacillus subtilis) | ||||||
| Activity: | Peptidylprolyl isomerase, with EC number 5.2.1.8 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR solution structure of B. subtilis PrsA PPIase
Overview
PrsA is a peptidyl-prolyl isomerase (PPIase) from Bacillus subtilis belonging to the parvulin family of PPIases. It is a membrane bound lipoprotein at the membrane-wall interface, involved in folding of exported proteins. We present the NMR solution structure of the PPIase domain of PrsA, the first from a Gram-positive bacterium. In addition we mapped out the active site with NMR titration experiments. A high degree of conservation with other members of the parvulin family was revealed in the structure and binding site. Interactions with substrate peptides were also characterized by mutated domains revealing that H122 is indispensable for overall correct folding.
About this Structure
1ZK6 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
NMR solution structure and characterization of substrate binding site of the PPIase domain of PrsA protein from Bacillus subtilis., Tossavainen H, Permi P, Purhonen SL, Sarvas M, Kilpelainen I, Seppala R, FEBS Lett. 2006 Mar 20;580(7):1822-6. Epub 2006 Feb 24. PMID:16516208
Page seeded by OCA on Thu Mar 20 15:36:29 2008
