3eo5

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[[Image:3eo5.png|left|200px]]
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==Crystal structure of the resuscitation promoting factor RpfB==
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<StructureSection load='3eo5' size='340' side='right' caption='[[3eo5]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3eo5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EO5 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xsf|1xsf]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eo5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eo5 RCSB], [http://www.ebi.ac.uk/pdbsum/3eo5 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/3eo5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mycobacterium tuberculosis is able to establish a non-replicating state and survive in an intracellular habitat for years. Resuscitation of dormant M. tuberculosis bacteria is promoted by resuscitation-promoting factors (Rpfs), which are secreted from slowly replicating bacteria close to dormant bacteria. Here we report the crystal structure of a truncated form of RpfB (residues 194-362), the sole indispensable Rpf of the five Rpfs encoded in this bacterium genome. The structure, denoted as (DeltaDUF)RpfB, exhibits a comma-like shape formed by a lysozyme-like globular catalytic domain and an elongated G5 domain, which is widespread among cell surface binding proteins. The G5 domain, whose structure was previously uncharacterised, presents some peculiar features. The basic structural motif of this domain, which represents the tail of the comma-like structure, is a novel super-secondary-structure element, made of two beta-sheets interconnected by a pseudo-triple helix. This intricate organisation leads to the exposure of several backbone hydrogen-bond donors/acceptors. Mutagenesis analyses and solution studies indicate that this protein construct as well as the full-length form are elongated monomeric proteins. Although (DeltaDUF)RpfB does not self-associate, the exposure of structural elements (backbone H-bond donors/acceptors and hydrophobic side chains) that are usually buried in globular proteins is typically associated with adhesive properties. This suggests that the RpfB G5 domain has a cell-wall adhesive function, which allows the catalytic domain to be properly oriented for the cleavage reaction. Interestingly, sequence comparisons indicate that these structural features are also shared by G5 domains involved in biofilm formation.
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{{STRUCTURE_3eo5| PDB=3eo5 | SCENE= }}
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Crystal structure of the resuscitation-promoting factor (DeltaDUF)RpfB from M. tuberculosis.,Ruggiero A, Tizzano B, Pedone E, Pedone C, Wilmanns M, Berisio R J Mol Biol. 2009 Jan 9;385(1):153-62. Epub 2008 Oct 22. PMID:18992255<ref>PMID:18992255</ref>
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===Crystal structure of the resuscitation promoting factor RpfB===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18992255}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3eo5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EO5 OCA].
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</StructureSection>
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Berisio, R.]]
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[[Category: Berisio, R]]
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[[Category: Pedone, C.]]
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[[Category: Pedone, C]]
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[[Category: Pedone, E.]]
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[[Category: Pedone, E]]
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[[Category: Ruggiero, A.]]
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[[Category: Ruggiero, A]]
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[[Category: Tizzano, B.]]
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[[Category: Tizzano, B]]
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[[Category: Wilmanns, M.]]
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[[Category: Wilmanns, M]]
[[Category: Cell adhesion]]
[[Category: Cell adhesion]]
[[Category: Crystal]]
[[Category: Crystal]]
[[Category: Resuscitation]]
[[Category: Resuscitation]]

Revision as of 13:19, 19 November 2014

Crystal structure of the resuscitation promoting factor RpfB

3eo5, resolution 1.83Å

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